Betula pendula Roth leaves: gastroprotective effects of an HPLC fingerprinted methanolic extract
| Autor: | Francesco Cacciola, Paola Dugo, Maria Paola Germanò, Luigi Mondello, Giovanna Certo, Antonio Rapisarda, Valeria D'Angelo, Paola Donato |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
gastroprotective activity
Plant Science Biochemistry High-performance liquid chromatography Analytical Chemistry Lipid peroxidation chemistry.chemical_compound Malondialdehyde Animals Stomach Ulcer Kaempferols Betula Chromatography High Pressure Liquid chemistry.chemical_classification Ethanol Chromatography Autoxidation Plant Extracts Methanol Organic Chemistry Glycoside Galactosides lipid peroxidation Anti-Ulcer Agents Rats Plant Leaves Betula pendula Roth leaves myricetin-3-O-galactoside chemistry Gastric Mucosa quercetin 3-O-galactoside Betula pendula Roth leaves gastroprotective activity lipid peroxidation quercetin 3-O-galactoside myricetin-3-O-galactoside Kaempferol Quercetin |
| Popis: | In this study, a methanolic extract of Betula pendula leaves (BLE) was investigated for its gastroprotective effects against 90% ethanol-induced ulcer in rats. Oral pretreatment of rats with BLE (100, 200 and 400 mg kg(- 1)) significantly reduced the incidence of gastric lesions induced by ethanol administration as compared with misoprostol (0.50 mg kg(- 1)). Furthermore, BLE inhibited the increase in malondialdehyde (MDA) and prevented depletion of total sulhydryl and non-protein sulhydryl groups in rat stomach homogenate when compared with ethanol group. With regard to the effect of lipid peroxidation in vitro, BLE showed the ability to reduce methyl linoleate autoxidation. Chemical characterisation of the main biologically active constituents of BLE was also achieved by means of high-performance liquid chromatography with photodiode array and mass spectrometry detection, showing the presence of myricetin-3-O-galactoside, quercetin glycosides, kaempferol glycosides. |
| Databáze: | OpenAIRE |
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