Generation and characterization of the human induced pluripotent stem cell line NMIi010-A from peripheral blood mononuclear cells of a healthy 49–year old male individual
| Autor: | Christian Schmees, Anna-Lena Keller, Ricarda Breitmeyer, Sabrina Vogel, Anna Binner, Nicole Anderle, Ulrich Rothbauer, Katja Schenke-Layland |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine QH301-705.5 Induced Pluripotent Stem Cells CD34 Germ layer Biology Peripheral blood mononuclear cell 03 medical and health sciences 0302 clinical medicine Humans Biology (General) Progenitor cell Induced pluripotent stem cell Comparative Genomic Hybridization Cell Differentiation Cell Biology General Medicine Middle Aged Cellular Reprogramming Cell biology Haematopoiesis 030104 developmental biology Cell culture Leukocytes Mononuclear Reprogramming 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 54, Iss, Pp 102427-(2021) |
| ISSN: | 1873-5061 |
| Popis: | Peripheral-blood derived CD34+ hematopoietic stem and progenitor cells were isolated from a 49-year old male donor and were successfully reprogrammed into human induced pluripotent stem cells (hiPSCs) using integration-free episomal vectors. The hiPSC line exhibited a typical stem cell-like morphology and endogenously expressed several pluripotency markers by concomitant loss of exogenous reprogramming vectors. Genomic integrity was confirmed by microarray-based comparative genomic hybridization (array CGH). Further analysis affirmed the ability of this hiPSC line to differentiate into all three germ layers. Thus, the reported cell line may serve as a healthy control for disease modeling. |
| Databáze: | OpenAIRE |
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