Diverse fragment lengths dismiss size selection for serum cell-free DNA: a comparative study of serum and plasma samples
| Autor: | Jiayi Mu, Shu Zheng, Yongmao Song, Yanqin Huang, Xuefeng Fang, Lina Qi, Weiting Ge, Ying Yuan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Base Sequence Plasma samples Chemistry Biochemistry (medical) Clinical Biochemistry Electrophoresis Capillary DNA Fragmentation General Medicine Serum samples Molecular biology Circulating Tumor DNA Plasma.cfDNA Highly sensitive Fragment size 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Capillary electrophoresis Cell-free fetal DNA 030220 oncology & carcinogenesis Area under curve Biomarkers Tumor Humans |
| Zdroj: | Clinical Chemistry and Laboratory Medicine (CCLM). 58:1451-1459 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm-2020-0078 |
| Popis: | Background The objective of this study was to determine the features of fragment length for circulating cell-free DNA (cfDNA) from plasma and serum samples. Methods Plasma and serum samples from different sources were randomly collected. Circulating cfDNA was extracted and purified by a precipitation-enriched and spin-column-based kit. The concentration of the purified DNA was immediately measured by a highly sensitive dsDNA quantitative assay, and then the fragment length was analyzed by capillary electrophoresis. The abundance of a specific fragment was estimated by the area under curve (AUC) for the fragment peak in the capillary electrophoresis. Results A total of 199 plasma and 117 serum samples were extracted and analyzed. The average yield of cfDNA from the serum samples (131.67 ng/mL) was significantly higher than that from the plasma samples (32.78 ng/mL, p Conclusions The fragment lengths of serum cfDNA and plasma cfDNA have very different features. Fragment size selection is suitable for plasma cfDNA but may not apply to serum cfDNA. |
| Databáze: | OpenAIRE |
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