Estrogen receptor antagonist fulvestrant inhibits proliferation and promotes apoptosis of prolactinoma cells by regulating the IRE1/XBP1 signaling pathway
| Autor: | Liang Chang, Guofu Li, Chao Wang, Xin Wang, Ming-Han Bai, Jun Su, Dongzhi Zhang, Chunlei Tan, Yuan Wen, Lingyu Xie |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
X-Box Binding Protein 1 endocrine system Cancer Research endocrine system diseases Cell Survival Cell Estrogen receptor Apoptosis Protein Serine-Threonine Kinases Biochemistry Flow cytometry 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Endoribonucleases Genetics medicine Humans Prolactinoma Viability assay Protein Precursors Molecular Biology Endoplasmic Reticulum Chaperone BiP Fulvestrant Heat-Shock Proteins Cell Proliferation medicine.diagnostic_test Chemistry Cell cycle Prolactin 030104 developmental biology medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis Cancer research Molecular Medicine Estrogen Receptor Antagonists hormones hormone substitutes and hormone antagonists medicine.drug Signal Transduction |
| Zdroj: | Molecular medicine reports. 18(4) |
| ISSN: | 1791-3004 |
| Popis: | The aim of the present study was to evaluate the effects of an estrogen receptor antagonist, fulvestrant, on proliferation and apoptosis of prolactinoma cells, and to reveal potential regulatory mechanisms. Prolactinoma GH3 cells were treated with 10‑6 mol/l fulvestrant for 2, 4, 8, 12 and 24 h. GH3 cell growth was observed under a microscope and cell viability was detected by MTT assay. Morphological changes of the nuclei in GH3 cells were observed by Hoechst 33258 staining and apoptotic rates were detected by flow cytometry. Preprolactin (PPL) and prolactin (PRL) secretion levels from GH3 cells were measured using ELISA. In addition, the protein expression levels of inositol‑requiring enzyme 1 (IRE1), X‑box binding protein (XBP)‑1 and glucose‑regulated protein, 78 kDa (GRP78) in GH3 cells were detected by western blot analysis. Cell density and cell viability of GH3 cells were significantly reduced in a time‑dependent manner following treatment with fulvestrant (P |
| Databáze: | OpenAIRE |
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