Human cytochrome b561: a revised hypothesis for conformation in membranes which reconciles sequence and functional information
| Autor: | Meera Srivastava, K. R. Gibson, Harvey B. Pollard, P. J. Fleming |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Cytochrome Protein Conformation Placenta Molecular Sequence Data Biology Kidney Biochemistry Complementary DNA Sequence Homology Nucleic Acid Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Peptide sequence Lung Pancreas Cytochrome b561 Base Sequence cDNA library Cytochrome b Cell Membrane Brain Cell Biology Cytochrome b Group Transmembrane protein Membrane topology biology.protein Cattle Research Article |
| Zdroj: | The Biochemical journal. 303 |
| ISSN: | 0264-6021 |
| Popis: | Cytochrome b561 is a major transmembrane protein of catecholamine and neuropeptide secretory vesicles. In this report, we describe the cloning and properties of a full-length cDNA encoding human neuroendocrine cytochrome b561 from a human caudate cDNA library and a human peripheral blood genomic library. The human cDNA contains two major transcription start sites and only one translation start site that codes for an apocytochrome b561, which is 22 amino acid residues smaller than the previously deduced amino acid sequence from bovine cDNA. This smaller version of cytochrome b561 may contain only five transmembrane segments rather than the previously proposed six segments. The new model is in agreement with our previous results on transmembrane topology of the gene product. Northern-blot analysis shows an expanded tissue distribution of cytochrome mRNA expression where previous immunological assays were negative. These results support the hypothesis that cytochrome b561 is a marker for peptidergic and adrenergic tissues. |
| Databáze: | OpenAIRE |
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