Synthesis of a Full-Length Infectious cDNA Clone of Cucurbit Aphid-Borne Yellows Virus and Its Use in Gene Exchange Experiments with Structural Proteins from Other Luteoviruses

Autor: Gérard Jonard, Hubert Guilley, Hervé Lecoq, Kenneth Richards, D. Prüfer, Catherine Wipf-Scheibel
Přispěvatelé: Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA)
Rok vydání: 1995
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
0106 biological sciences
DNA
Complementary
viruses
Molecular Sequence Data
Luteovirus
01 natural sciences
Virus
Open Reading Frames
INTERACTION VIRUS VECTEUR
03 medical and health sciences
Virology
Complementary DNA
Escherichia coli
medicine
Animals
T7 RNA polymerase
Cloning
Molecular
Gene
DNA Primers
030304 developmental biology
Viral Structural Proteins
2. Zero hunger
Genetics
0303 health sciences
Base Sequence
biology
Protoplasts
fungi
Virion
food and beverages
RNA
biology.organism_classification
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
3. Good health
Aphids
DNA
Viral
Mutation
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
RNA
Viral
Cauliflower mosaic virus
Myzus persicae
010606 plant biology & botany
medicine.drug
Zdroj: Virology
Virology, Elsevier, 1995, 214, pp.150-158. ⟨10.1006/viro.1995.9945⟩
ISSN: 0042-6822
1096-0341
Popis: International audience; A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35S promoter in an agroinfection vector. The biological activity of the cloned cDNA was demonstrated by the ability of its in vitro transcript to replicate in protoplasts and of the agroinfection vector to infect agroinoculated plants. Virus in the agroinfected plants could be transmitted by the aphid vectors Myzus persicae and Aphis gossypii. The specificity of luteovirus RNA packaging was investigated by replacing (1) the CABYV coat protein gene (and the overlapping ORF5) by the corresponding region of potato leafroll luteovirus or (2) the CABYV readthrough domain by the readthrough domain of beet western yellows luteovirus. The resulting chimeric transcripts replicated in protoplasts and produced virions.
Databáze: OpenAIRE
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