Lipid Composition and Barrier Function of Human Skin after Grafting onto Athymic Nude Mice
| Autor: | Harry E. Boddé, Braham Shroot, Maria Ponec, Michel Demarchez, Hans Schaefer, F. Spies, Isabelle Higounenc |
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| Rok vydání: | 1994 |
| Předmět: |
Tritiated water
Physiology Ratón Skin Absorption Lipid composition Transplantation Heterologous Mice Nude Human skin Thymus Gland Dermatology In Vitro Techniques Ceramides Mice chemistry.chemical_compound Skin Physiological Phenomena Animals Freeze Fracturing Humans Phospholipids Barrier function Skin Pharmacology Chemistry Skin Transplantation General Medicine Penetration (firestop) Lipid Metabolism Lipids Water Loss Insensible Molecular biology Transplantation Immunology Chromatography Thin Layer |
| Zdroj: | Skin Pharmacology and Physiology. 7:167-175 |
| ISSN: | 1660-5535 1660-5527 |
| DOI: | 10.1159/000211291 |
| Popis: | The barrier properties of human epidermis grafted for 1–3 months onto nude mice are compared with normal human skin. Beside penetration studies with tritiated water and measurements of transepidermal water loss (TEWL), we analyzed the epidermal lipids by high-performance thin layer chromatography and evaluated the ultrastructure of the intercorneocyte lipid arrangement by freeze fracture electron microscopy (FFEM). The permeability of human skin for tritiated water and the TEWL exhibit no significant changes after grafting onto nude mice. FFEM analysis showed that grafted epidermis has the same morphological pattern as normal human epidermis. Regular desmosomes and lamellar lipid structures are present. Grafting did not qualitatively affect the lipid composition of human epidermis. Ceramides which contribute largely to the barrier function, have the same distribution profile. |
| Databáze: | OpenAIRE |
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