Modeling membrane morphological change during autophagosome formation
| Autor: | Masashi Tachikawa, Roland L. Knorr, Noboru Mizushima, Ikuko Koyama-Honda, Yuji Sakai |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Autophagosome Biophysics Membrane Architecture FOS: Physical sciences 02 engineering and technology Condensed Matter - Soft Condensed Matter Curvature Article Quantitative Biology::Subcellular Processes 03 medical and health sciences Physics - Biological Physics Degradation process lcsh:Science Molecular Biology Multidisciplinary Chemistry Autophagy Cell Biology 021001 nanoscience & nanotechnology Autophagosome formation 030104 developmental biology Membrane Membrane curvature Biological Physics (physics.bio-ph) Soft Condensed Matter (cond-mat.soft) lcsh:Q sense organs 0210 nano-technology Intracellular |
| Zdroj: | iScience, Vol 23, Iss 9, Pp 101466-(2020) iScience |
| DOI: | 10.48550/arxiv.2009.01545 |
| Popis: | Summary Autophagy is an intracellular degradation process that is mediated by de novo formation of autophagosomes. Autophagosome formation involves dynamic morphological changes; a disk-shaped membrane cisterna grows, bends to become a cup-shaped structure, and finally develops into a spherical autophagosome. We have constructed a theoretical model that integrates the membrane morphological change and entropic partitioning of putative curvature generators, which we have used to investigate the autophagosome formation process quantitatively. We show that the membrane curvature and the distribution of the curvature generators stabilize disk- and cup-shaped intermediate structures during autophagosome formation, which is quantitatively consistent with in vivo observations. These results suggest that various autophagy proteins with membrane curvature-sensing properties control morphological change by stabilizing these intermediate structures. Our model provides a framework for understanding autophagosome formation. Graphical Abstract Highlights • A model of morphological transition during autophagosome formation is established • A dynamic distribution of curvature generators stabilizes cup-shaped intermediates • An abundance of curvature generators regulates the size of autophagosomes • The model is quantitively consistent with in vivo observations Biophysics; Cell Biology; Molecular Biology; Membrane Architecture |
| Databáze: | OpenAIRE |
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