Transcriptomic profiling of neural stem cell differentiation on graphene substrates

Autor: Yangnan Hu, Yuhua Zhang, Qilin Tang, Mingliang Tang, Menghui Liao, Xiaoqian Yan, Li He, Rongrong Guo, Lin Xia, Renjie Chai, Buwei Shao, Yun Liu, Dan Li, Xing Guo, Jian Li
Rok vydání: 2018
Předmět:
Candidate gene
Cell
Primary Cell Culture
02 engineering and technology
01 natural sciences
Regenerative medicine
Hippocampus
Transcriptome
Mice
Colloid and Surface Chemistry
Tissue engineering
Neural Stem Cells
Tubulin
0103 physical sciences
Glial Fibrillary Acidic Protein
Protein Interaction Mapping
medicine
Animals
Physical and Theoretical Chemistry
Gene
reproductive and urinary physiology
010304 chemical physics
Chemistry
Gene Expression Profiling
Computational Biology
Gene Expression Regulation
Developmental
Cell Differentiation
Surfaces and Interfaces
General Medicine
Axonemal Dyneins
021001 nanoscience & nanotechnology
Embryo
Mammalian
Neural stem cell
Cell biology
medicine.anatomical_structure
nervous system
Cell culture
Polystyrenes
Graphite
0210 nano-technology
Biotechnology
Signal Transduction
Zdroj: Colloids and surfaces. B, Biointerfaces. 182
ISSN: 1873-4367
Popis: Graphene exhibits excellent mechanical strength, electrical conductivity and good biocompatibility, which make it a suitable candidate as a neural interfacing material in regenerative medicine and tissue engineering. Graphene is reported to promote both of neural stem cells (NSCs) proliferation and differentiation. However, the transcriptomes of 2D graphene-regulated NSC differentiation have not yet been investigated. To identify candidate genes, on which graphene may affect, we used next-generation RNA sequencing to analyze the transcriptome of NSCs differentiated for 21 days on a graphene substrate. These NSCs displayed highly enriched and differentially expressed genes compared with traditional cell culture in vitro. Of these, we identified motor protein genes that might regulate NSC differentiation, including cytoplasmic dynein and axonemal dynein genes, Ccdc108, Dnah5, and Dnah11. Furthermore, we analyzed the cell signaling pathway genes that might regulate NSC differentiation, and we constructed a protein-protein interaction network for the genes that are differentially expressed in NSCs on graphene compared to commercial tissue culture polystyrene substrates. We have identified genes potentially regulating the differentiation and migration of NSCs on graphene substrates, and our findings provide mechanistic evidence for the biological activities of graphene, especially in view of graphene-stem cell interactions.
Databáze: OpenAIRE
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