A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method

Autor: Vanessa Fey Pascoal, Malcolm K. Jones, Catieli Gobetti Lindholz, Renata Perotto de Souza, Hélio Radke Bittencourt, Angela Regina Piovesan, Carlos Graeff-Teixeira, Carolina De Marco Verissimo, Francine de Vargas Rigo, Célia R. Carlini, Alessandra Loureiro Morassutti, Vivian Favero, André Arigony Souto
Rok vydání: 2020
Předmět:
0301 basic medicine
Ascaris Lumbricoides
Veterinary medicine
Schistosoma Mansoni
Nematoda
Light
Physiology
Eggs
RC955-962
Luminol
law.invention
Feces
Mice
chemistry.chemical_compound
Medical Conditions
0302 clinical medicine
Reproductive Physiology
law
Arctic medicine. Tropical medicine
Medicine and Health Sciences
Schistosomiasis
Sedimentary Geology
biology
Physics
Electromagnetic Radiation
Ascaris
Eukaryota
Geology
Diagnostic strategy
Infectious Diseases
Helminth Infections
Physical Sciences
Schistosoma
Light emission
Schistosoma mansoni
Public aspects of medicine
RA1-1270
Research Article
Neglected Tropical Diseases
030231 tropical medicine
03 medical and health sciences
Helminths
Parasitic Diseases
medicine
Animals
Humans
Petrology
Ovum
Chemiluminescence
Organisms
Public Health
Environmental and Occupational Health
Biology and Life Sciences
Hydrogen Peroxide
Tropical Diseases
biology.organism_classification
medicine.disease
Invertebrates
Schistosomiasis mansoni
030104 developmental biology
chemistry
Luminescent Measurements
Earth Sciences
Sediment
Zoology
Zdroj: PLoS Neglected Tropical Diseases
PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008500 (2020)
ISSN: 1935-2735
DOI: 10.1371/journal.pntd.0008500
Popis: Schistosomiasis remains a serious public health problem in tropical regions, affecting more than 250 million people. Sensitive diagnostic methods represent key tools for disease elimination, in particular in areas with low endemicity. Advances in the use of luminol-based chemiluminescent techniques have enabled greater sensitivity and speed in obtaining results in different diagnostic settings. In this study, we developed a luminol-H2O2 chemiluminescence (CL) method to detect Schistosoma mansoni eggs in human fecal sediments processed by the Helmintex (HTX) method. After S. mansoni eggs were incubated with a solution of luminol-H2O2 the light emission was detected and measured by spectrophotometry at 431 nm for 5 min, using detection and counts of eggs by bright field optical microscopy as a reference. CL intensity was found to correlate with different sources and numbers of eggs. Furthermore, our results showed that the CL method can distinguish positive from negative samples with 100% sensitivity and 71% specificity. To our knowledge, this is the first study to report the use of CL for the diagnosis of helminths from fecal samples. The combination of the HTX method with CL represents an important advance in providing a reference method with the highest standards of sensitivity.
Author summary Schistosomiasis, the infection caused by parasites of the genus Schistosoma, is still a significant health problem in many countries, despite some successful control efforts. In areas where parasite load is low and few eggs are released in feces, most diagnostic tests fail to detect the infections. Helmintex is a very sensitive, but time consuming, egg-detection method, with most time being consumed in observations of samples at the microscope. Here we report standardization of an innovative luminol-H2O2 chemiluminescence reaction triggered by Schistosoma mansoni eggs that may improve their detection in feces. Improved diagnostic tests are urgently required as part of the schistosomiasis elimination efforts.
Databáze: OpenAIRE
Externí odkaz: