A pilot study identifying a set of microRNAs as precise diagnostic biomarkers of acute kidney injury
| Autor: | Macarena Rodriguez, Elisa Conde, Aurora Lietor, Angel Candela, Edurne Ramos, Laura Martín-Gómez, Fernando Liaño, Belen Ponte, Elia Aguado-Fraile, María Laura García-Bermejo |
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| Přispěvatelé: | Instituto de Salud Carlos III, Comunidad de Madrid, Fundación Alfonso Martín Escudero, Red Española de Investigación Renal |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Genetic Markers Male lcsh:Medicine Pilot Projects Bioinformatics chemistry.chemical_compound Intensive care microRNA medicine Diagnostic biomarker Humans Biomarker discovery Cardiac Surgical Procedures lcsh:Science ddc:616 Creatinine Multidisciplinary business.industry Gene Expression Profiling lcsh:R Acute kidney injury Acute Kidney Injury Middle Aged medicine.disease Gene expression profiling MicroRNAs chemistry ROC Curve Creatinine increased lcsh:Q Female business Research Article |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname PLoS ONE PLoS ONE, Vol 10, Iss 6, p e0127175 (2015) PLOS ONE, Vol. 10, No 6 (2015) P. e0127175 Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid Plos One, vol. 10, no. 6, pp. e0127175 |
| ISSN: | 1932-6203 |
| Popis: | © 2015 Aguado-Fraile et al. In the last decade, Acute Kidney Injury (AKI) diagnosis and therapy have not notably improved probably due to delay in the diagnosis, among other issues. Precocity and accuracy should be critical parameters in novel AKI biomarker discovery. microRNAs are key regulators of cell responses to many stimuli and they can be secreted to the extracellular environment. Therefore, they can be detected in body fluids and are emerging as novel disease biomarkers. We aimed to identify and validate serum miRNAs useful for AKI diagnosis and management. Using qRT-PCR arrays in serum samples, we determined miRNAs differentially expressed between AKI patients and healthy controls. Statistical and target prediction analysis allowed us to identify a panel of 10 serum miRNAs. This set was further validated, by qRT-PCR, in two independent cohorts of patients with relevant morbi-mortality related to AKI: Intensive Care Units (ICU) and Cardiac Surgery (CS). Statistical correlations with patient clinical parameter were performed. Our results demonstrated that the 10 selected miRNAs (miR-101-3p, miR-127-3p, miR-210-3p, miR-126-3p, miR-26b-5p, miR-29a-3p, miR-146a-5p, miR-27a-3p, miR-93-3p and miR-10a-5p) were diagnostic biomarkers of AKI in ICU patients, exhibiting areas under the curve close to 1 in ROC analysis. Outstandingly, serum miRNAs estimated before CS predicted AKI development later on, thus becoming biomarkers to predict AKI predisposition. Moreover, after surgery, the expression of the miRNAs was modulated days before serum creatinine increased, demonstrating early diagnostic value. In summary, we have identified a set of serum miRNAs as AKI biomarkers useful in clinical practice, since they demonstrate early detection and high diagnostic value and they recognize patients at risk. This work was supported by grants FIS PS09/02183 and PS12/00094 by Instituto de Salud Carlos III (ISCIII) and Comunidad de MAdrid-Consorcio de Investigación en Fracaso Renal Agudo (CAM-CIFRA, S2010/BMD-2378). EAF was supported by CAM-CIFRA (S2010/BMD-2378) and is currently funded by Eusebio Martín Escudero Foundation Fellowship, ER was funded by Red de Investigación Renal (REDINREN, RD12/0021/0020), MR was recipient of contract from ISCIII (CA11/00491). |
| Databáze: | OpenAIRE |
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