Changes in the Inner Retinal Cells after Intense and Constant Light Exposure in Sprague-Dawley Rats
| Autor: | Tapas Chandra Nag, Tara Sankar Roy, Pankaj Kumar, Meenakshi Maurya, Sneha Gupta, Kumar Abhiram Jha, Poorti Kathpalia, Chandan Lal Gupta |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Light Mice Transgenic Biochemistry Retina Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Downregulation and upregulation Recoverin Internal medicine Glial Fibrillary Acidic Protein medicine Sprague dawley rats Animals Photoreceptor Cells Rats Long-Evans Physical and Theoretical Chemistry Constant light biology Chemistry Retinal Dose-Response Relationship Radiation General Medicine Ganglion Rats 030104 developmental biology medicine.anatomical_structure Endocrinology 030221 ophthalmology & optometry biology.protein Female sense organs Parvalbumin |
| Zdroj: | Photochemistry and photobiologyReferences. 96(5) |
| ISSN: | 1751-1097 |
| Popis: | Light insult causes photoreceptor death. Few studies reported that continuous exposure to light affects horizontal, Muller and ganglion cells. We aimed to see the effect of constant light exposure on bipolar and amacrine cells. Adult Sprague-Dawley rats were exposed to 300 or 3000 lux for 7 days in 12-h light: 12-h dark cycles (12L:12D). The latter group was then exposed to 24L:0D for 48 h to induce significant damage. The same animals were reverted to 300 lux and reared for 15 days in 12L:12D cycles. They were sacrificed on different days to find the degree of retinal recovery, if any, from light injury. Besides photoreceptor death, continuous light for 48 h resulted in downregulation of parvalbumin in amacrine cells and recoverin in cone bipolar cells (CBC). Rod bipolar cells (RBC) maintained an unaltered pattern of PKC-α expression. Upon reversal, there were increased expressions of parvalbumin in amacrine cells and recoverin in CBC, while RBC showed an increasing trend of PKC-α expression. The data show that damage in bipolar and amacrine cells after exposure to intense, continuous light can be ameliorated upon reversal to normal LD cycles to which the animals were initially acclimated to. |
| Databáze: | OpenAIRE |
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