Fluorescein-concanavalin A conjugates distinguish between normal and malignant human cells: a preliminary report
| Autor: | C. J. Louis, R. G. Wyllie |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Pathology
medicine.medical_specialty Lung Neoplasms Skin Neoplasms Cell Fluorescent Antibody Technique Breast Neoplasms Stain Cellular and Molecular Neuroscience chemistry.chemical_compound Preliminary report Neoplasms medicine Concanavalin A Humans Fluorescein Molecular Biology Skin Pharmacology biology Liver Neoplasms Cell Biology Fluoresceins Fluorescence Pancreatic Neoplasms medicine.anatomical_structure Membrane chemistry biology.protein Molecular Medicine Female Fluorescein-5-isothiocyanate Thiocyanates Conjugate |
| Zdroj: | Experientia. 37(5) |
| ISSN: | 0014-4754 |
| Popis: | A method is described for using a fluorescein isothiocyanate concanavalin A conjugate to stain human cell membranes in formalin fixed paraffin embedded tissue. 57 neoplastic and normal tissue sites were examined. In 34 malignant tumours, bright green fluorescence was confined to the cell membranes while in 23 benign tumours and normal tissue sites, the membranes were unstained or showed a diminished level of fluorescence. The distinction between malignant and hyperplastic or normal cells was clear cut and definite. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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