Detection of porcine circovirus type 2 (PCV2) in the Philippines and the complexity of PCV2-associated disease diagnosis
| Autor: | M. R. Mananggit, Christina Legaspi, C. R. Parke, Tamsin S. Barnes, Rachel R. Azul, C. Ignacio, John I. Alawneh, Emmanuel Francisco, Voltaire G. Basinang, E. L. Lapuz, Ronnie Domingo, Conny Turni, Agnes dela Cruz, J. E. David, Patrick J. Blackall, E. C. Villar, Suzan Lola, Joanne Meers, Chiara Palmieri, A. S. Baluyut, Ronilo O. de Castro, Lilia M. Retes |
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| Rok vydání: | 2021 |
| Předmět: |
Circovirus
medicine.medical_specialty Pathology Swine 040301 veterinary sciences Philippines animal diseases Spleen Antibodies Viral 0403 veterinary science Food Animals Antigen medicine Animals Circoviridae Infections Pathogen Swine Diseases biology 0402 animal and dairy science virus diseases 04 agricultural and veterinary sciences biology.organism_classification 040201 dairy & animal science Porcine circovirus Real-time polymerase chain reaction medicine.anatomical_structure Immunohistochemistry Animal Science and Zoology Histopathology Lymph Nodes Lymph |
| Zdroj: | Tropical Animal Health and Production. 53 |
| ISSN: | 1573-7438 0049-4747 |
| Popis: | Porcine circovirus type 2 (PCV2), an important pig viral pathogen, can cause porcine circovirus-associated disease (PCVAD), resulting in economic losses associated with decreased growth and mortalities. The diagnosis of PCVAD is complex requiring clinical, pathological and virological approaches. This study assessed PCV2 infection using histopathology and immunohistochemistry (IHC) on tissue samples and quantitative polymerase chain reaction (qPCR) on serum samples from 47 grower-finisher pigs allocated in three clinical groups in the Philippines. Typical PCV2 histopathological lesions were observed in mediastinal lymph nodes (MLN) of eight of 47 pigs. Lymphoid depletion was seen in all eight pigs and granulomatous inflammation in one of these pigs. Four of these eight pigs were PCV2 positive by both IHC and qPCR. IHC revealed PCV2 antigen in 8 pigs in at least one of the following tissues: MLN (5/8), spleen (3/8), tonsils (4/8) and lungs (5/8). PCV2 antigen was observed in 3/8 MLN with lymphoid depletion and in one MLN with depletion and granulomatous inflammation. The qPCR test showed that 33 sera had a non-detectable level, twelve had 106 PCV2 DNA copies/ml serum. One pig with lymphoid depletion had > 106 PCV2 DNA copies/ml serum, and another pig without MLN lesions also had > 106 PCV2 DNA copies/ml serum. These findings suggest that PCVAD is present in the Philippines and confirm the challenges of PCVAD diagnosis as different patterns of results were obtained from the different tests. |
| Databáze: | OpenAIRE |
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