Nickel oxide nanoparticles induced DNA damages in human liver cells
| Autor: | Gül Özhan, Mahmoud Abudayyak, Emine Güzel Meydanli |
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| Rok vydání: | 2021 |
| Předmět: |
Neutral red
Cytotoxicity Intratracheal Instillation Apoptosis medicine.disease_cause chemistry.chemical_compound medicine Propidium iodide Nickel oxide Nanotoxicology Toxicity Farmakoloji ve Eczacılık Acute Oxidative Stress Nickel oxide Nanoparticles Genotoxicity Oxidative stress Apoptosis Molecular biology Nio Comet assay chemistry Oxidative stress In-Vitro Nanoparticles Comet Assay Genotoxicity Pharmacology and Pharmacy |
| Zdroj: | Volume: 51, Issue: 2 175-182 İstanbul Journal of Pharmacy |
| ISSN: | 2587-2087 |
| Popis: | Background and Aims: Nickel oxide nanoparticles (NiO-NPs) are one of the most used nanoparticles, especially as photosensitizers. Although some studies evaluate their toxicity in the liver, the information about their toxicity at the cellular and molecular levels is still controversial. In the present study, it was aimed to investigate the in vitro toxic potentials of NiO-NPs (average size 15.0 nm) in the liver (HepG2) cell Line. Methods: NiO-NPs were characterized by Transmission Electron Microscopy (TEM), the cellular uptake of NPs and the morphologic changes were evaluated by TEM and Inductively Coupled Plasma-Mass Spectrometry (ICP-MS), the cytotoxicity was evaluated by MTT and neutral red uptake (NRU) tests, comet assay was used for genotoxicity, Annexin V-FITC/propidium iodide (PI) apoptosis detection kit was used for apoptosis/ necrosis evaluation and Enzyme-Linked Immune Sorbent Assays (ELISA) kits were used for the potential of oxidative damage. Results: Our results showed that cellular uptake of NiO-NPs led to morphological changes in the cells, and caused cell death (1050 was 146.7 mu g/mL by MTT) mainly by apoptosis. Genotoxicity and oxidative damage were observed to be in a dosedependent manner. Conclusion: Results confirm previous data and draw attention to the toxic effects of NiO-NPs; further in vivo and in vitro studies need to be done to clarify the safety or toxicity of NiO-NPs. Research Fund of Istanbul UniversityIstanbul University [37785] This work was supported by the Research Fund of Istanbul University (Project No: 37785) . |
| Databáze: | OpenAIRE |
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