Characteristics of Sulfobacin A from a Soil Isolate Chryseobacterium gleum
Autor: | Bhushan L. Chaudhari, Kishor S. Wani, S. B. Chincholkar, Priti N. Chaudhari |
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Rok vydání: | 2008 |
Předmět: |
Bioengineering
Polyenes Chryseobacterium gleum Biology Applied Microbiology and Biotechnology Biochemistry Serine chemistry.chemical_compound Biosynthesis Cell Line Tumor RNA Ribosomal 16S Anticarcinogenic Agents Humans Transition Temperature Molecular Biology Phylogeny Soil Microbiology Cell Proliferation Chryseobacterium chemistry.chemical_classification Pigmentation Spectrum Analysis Sequence Analysis DNA General Medicine Ribosomal RNA 16S ribosomal RNA biology.organism_classification Enzyme Alkanesulfonic Acids chemistry Cycloserine Cell culture Bacteria Biotechnology |
Zdroj: | Applied Biochemistry and Biotechnology. 158:231-241 |
ISSN: | 1559-0291 0273-2289 |
Popis: | A nonmotile, nonspore-forming, Gram-negative, aerobic, small rod-shaped bacterium, isolated from soil, was identified as Chryseobacterium gleum on the basis of 16S rRNA gene sequence analysis. It was observed to grow luxuriously at pH 9 and tolerate highly alkaline environment up to pH 12. Orange red color was a peculiar character of these cells which on purification obtained 60-80 mg/l and found to be sphingosine type of sulfonolipid "sulfobacin A" on the basis of infrared, nuclear magnetic resonance, and mass spectral data. Inhibition of sulfobacin A synthesis by incorporation of L: -cycloserine in culture growth medium suggested presence of serine palmitoyl transferase which is one of the important enzymes involved in its biosynthesis. Sulfobacin A from C. gleum LMG P-22264 exhibited cytotoxicity against four cell lines tested. Maximum activity against human mammary adenocarcinoma cells was indicative of its potential as an anticancer agent. |
Databáze: | OpenAIRE |
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