Longitudinal evaluation of herpes simplex virus DNA load during episodes of herpes labialis
| Autor: | Sharon Keays, Nathalie Goyette, Yan Sergerie, Guy Boivin, Tristan Booth |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
viruses Herpesvirus 1 Human Biology medicine.disease_cause Polymerase Chain Reaction Herpesviridae Virus Virology Chlorocebus aethiops medicine Animals Humans Viral shedding Vero Cells Herpes Labialis Herpes Simplex Viral Load Virus Shedding Infectious Diseases Real-time polymerase chain reaction Herpes simplex virus DNA Viral Immunology Female Viral disease Viral load |
| Zdroj: | Journal of Clinical Virology. 37:248-251 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2006.09.006 |
| Popis: | Background Few studies have performed sequential evaluation of the herpes simplex virus (HSV) load using quantitative PCR during episodes of herpes labialis. Objective To determine HSV viral load kinetics during recurrences of herpes labialis. Study design Twenty-two subjects were monitored by daily swabs during recurrences of herpes labialis. A real-time PCR detecting both HSV-1 and HSV-2 was used to quantify the viral load. Results Median duration of HSV-1 shedding was 60 h and 48 h by PCR and culture, respectively. No HSV-2 DNA was detected in that study. Peak viral DNA load (123.6 copies/cell) occurred at 48 h with no virus detected beyond 96 h of onset of symptoms. Conclusions These viral load kinetics data could be used as surrogate markers of antiviral activity in future clinical trials. |
| Databáze: | OpenAIRE |
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