An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
Autor: | Mario V. Jaramillo, Chunyu Wang, Jing Zhao, Hongmin Li, Kenneth V. Mills, Zhong Li, Jennie E. Williams |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Models
Molecular 0301 basic medicine Protein Conformation Stereochemistry Archaeal Proteins Science Gene Expression Biochemistry Article Homing endonuclease Inteins 03 medical and health sciences Pyrococcus horikoshii Protein Domains Protein splicing Thermolysin Protein Interaction Domains and Motifs Amino Acid Sequence Multidisciplinary 030102 biochemistry & molecular biology biology Chemistry Alternative splicing biology.organism_classification 030104 developmental biology biology.protein Medicine Structural biology Intein Linker Pyrococcus abyssi |
Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Protein splicing is a post-translational process by which an intein catalyzes its own excision from flanking polypeptides, or exteins, concomitant with extein ligation. Many inteins have nested homing endonuclease domains that facilitate their propagation into intein-less alleles, whereas other inteins lack the homing endonuclease (HEN) and are called mini-inteins. The mini-intein that interrupts the DNA PolII of Pyrococcus horikoshii has a linker region in place of the HEN domain that is shorter than the linker in a closely related intein from Pyrococcus abyssi. The P. horikoshii PolII intein requires a higher temperature for catalytic activity and is more stable to digestion by the thermostable protease thermolysin, suggesting that it is more rigid than the P. abyssi intein. We solved a crystal structure of the intein precursor that revealed a domain-swapped dimer. Inteins found as domain swapped dimers have been shown to promote intein-mediated protein alternative splicing, but the solved P. horikoshii PolII intein structure has an active site unlikely to be catalytically competent. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |