Maternal exposure to a continuous 900-MHz electromagnetic field provokes neuronal loss and pathological changes in cerebellum of 32-day-old female rat offspring
| Autor: | Orhan Baş, Ali Aslan, Hatice Hancı, Arzu Şahin, Haydar Kaya, Ayşe İkinci, Ersan Odaci, Serdar Colakoglu, Osman Sonmez |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cerebellum animal structures Offspring Stereology Purkinje cell Physiology Cell Count Biology Rats Sprague-Dawley Purkinje Cells 03 medical and health sciences Cellular and Molecular Neuroscience Electromagnetic Fields 0302 clinical medicine Pregnancy Electromagnetic field medicine Animals Prenatal exposure Pathological Neurons Age Factors Anatomy medicine.disease Female rat Rats 030104 developmental biology medicine.anatomical_structure Maternal Exposure Prenatal Exposure Delayed Effects Female 030217 neurology & neurosurgery Pyknosis |
| Zdroj: | Journal of Chemical Neuroanatomy. 75:105-110 |
| ISSN: | 0891-0618 |
| Popis: | WOS: 000381842500010 PubMed: 26391347 Large numbers of people are unknowingly exposed to electromagnetic fields (EMF) from wireless devices. Evidence exists for altered cerebellar development in association with prenatal exposure to EMF. However, insufficient information is still available regarding the effects of exposure to 900 megahertz (MHz) EMF during the prenatal period on subsequent postnatal cerebellar development. This study was planned to investigate the 32-day-old female rat pup cerebellum following exposure to 900 MHz EMF during the prenatal period using stereological and histopathological evaluation methods. Pregnant rats were divided into control, sham and EMF groups. Pregnant EMF group (PEMFG) rats were exposed to 900 MHz EMF for 1 h inside an EMF cage during days 13-21 of pregnancy. Pregnant sham group (PSG) rats were also placed inside the EMF cage during days 13-21 of pregnancy for 1 h, but were not exposed to any EMF. No procedure was performed on the pregnant control group (PCG) rats. Newborn control group (CG) rats were obtained from the PCG mothers, newborn sham group (SG) rats from the PSG and newborn EMF group (EMFG) rats from the PEMFG rats. The cerebellums of the newborn female rats were extracted on postnatal day 32. The number of Purkinje cells was estimated stereologically, and histopathological evaluations were also performed on cerebellar sections. Total Purkinje cell numbers calculated using stereological analysis were significantly lower in EMFG compared to CG (p < 0.05) and SG (p < 0.05). Additionally, some pathological changes such as pyknotic neurons with dark cytoplasm were observed in EMFG sections under light microscopy. In conclusion, our study results show that prenatal exposure to EMF affects the development of Purkinje cells in the female rat cerebellum and that the consequences of this pathological effect persist after the postnatal period. (C) 2015 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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