Ethanol as a potential mosquito sample storage medium for RNA preservation
| Autor: | Allison M Weakley, Oscar D. Kirstein, James D. Hibbert, Yoosook Lee, Mirsha G. Torres, Gregory C. Lanzaro |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sample (material) Clinical Sciences Oncology and Carcinogenesis Preservation Biological RNA preservation mosquito genetic analysis Mosquito Vectors Sample storage 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Rare Diseases field settings RNA analysis Genetics Animals General Pharmacology Toxicology and Pharmaceutics Malaria vector General Immunology and Microbiology Ethanol business.industry Brief Report RNA General Medicine Articles Biological Preservation Biotechnology Malaria Vector-Borne Diseases 010602 entomology 030104 developmental biology Infectious Diseases Good Health and Well Being Culicidae Environmental science Biochemistry and Cell Biology business |
| Zdroj: | F1000Research |
| ISSN: | 2046-1402 |
| Popis: | Sample storage for downstream RNA analysis can be challenging in some field settings, especially where access to cryogenic materials or refrigeration/freezer facilities are limited. This has limited RNA-based studies on African malaria vectors collected in the field. We evaluated RNA quality after storing mosquito samples in three different sample preservation media over a 4-week period. Storing mosquito specimens in cold (4°C) media significantly improved yields of intact RNA. Our results indicate commercially available products perform well in keeping RNA integrity as advertised. Moreover, absolute ethanol may be an economical alternative for sample preservation that can be utilized in some resource-limited settings. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|