Lipid Thermotropic Transitions in Human Stratum Corneum
| Autor: | Russell O Potts, James E McKie, R. R. Harris, Guia M Golden, Donald B. Guzek |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Calorimetry
Differential Scanning Spectrophotometry Infrared integumentary system Infrared Chemistry Cell Biology Dermatology Calorimetry Mass spectrometry Lipids Biochemistry Thermotropic crystal Differential scanning calorimetry Membrane medicine.anatomical_structure Stratum corneum medicine Biophysics Humans lipids (amino acids peptides and proteins) Epidermis Fourier transform infrared spectroscopy Molecular Biology |
| Zdroj: | Journal of Investigative Dermatology. (3):255-259 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12285373 |
| Popis: | The techniques of differential scanning calorimetry (DSC) and thermal perturbation infrared (IR) spectrometry were used to investigate thermal transitions in intact, fractionated, and lipid-extracted human stratum corneum. The DSC results show 3 major and one minor thermal transition in the range of 30-120 degrees C. Of particular interest to this study are 2 transitions seen near 65 degrees and 75 degrees C in intact stratum corneum and a stratum corneum membrane preparation, but absent from lipid-extracted samples. Results of IR spectrometry show that thermally induced spectral changes related to enhanced motion of the lipid acyl chains also occur in the region of about 60-80 degrees C. The combined DSC and IR results show that the thermal transitions near 65 degrees and 75 degrees C depend on water concentration in a manner identical to that seen for a variety of lipid-water systems. Taken together, these results suggest that thermal transitions occurring near 65 degrees and 85 degrees C involve increased acyl chain motion of stratum corneum lipids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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