Evaluation of the pro-angiogenic effect of nanoscale extracellular vesicles derived from human umbilical cord mesenchymal stem cells
| Autor: | Gabriel Dostert, Sébastien Hupont, Patrick Menu, Véronique Decot, Emilie Velot, Huguette Louis, Anne-Sophie Willemin, Pierre Gillet, Valérie Jouan-Hureaux, Vanessa Moby |
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| Přispěvatelé: | Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Centre de Recherche en Automatique de Nancy (CRAN), Bioingénierie Moléculaire, Cellulaire et Thérapeutique (BMCT), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Faculté de Pharmacie [Nancy], Université de Lorraine (UL) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Angiogenesis Cell Culture Techniques Regenerative medicine Umbilical cord Umbilical vein law.invention Umbilical Cord angiogenesis 0302 clinical medicine law MESH: Angiogenesis Inducing Agents MESH: Human Umbilical Vein Endothelial Cells Cells Cultured HUVECs medicine.diagnostic_test Chemistry General Medicine Cord lining 3. Good health Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis cardiovascular system MESH: Neovascularization Physiologic MESH: Cells Cultured Biomedical Engineering Neovascularization Physiologic [SDV.CAN]Life Sciences [q-bio]/Cancer Flow cytometry Biomaterials 03 medical and health sciences Extracellular Vesicles Confocal microscopy Nanoscale extracellular vesicles medicine Human Umbilical Vein Endothelial Cells Humans MESH: Particle Size Particle Size MESH: Extracellular Vesicles MESH: Cell Culture Techniques MESH: Humans MESH: Umbilical Cord Mesenchymal stem cell Mesenchymal Stem Cells human umbilical cord MSCs 030104 developmental biology MESH: Mesenchymal Stromal Cells Angiogenesis Inducing Agents |
| Zdroj: | Bio-Medical Materials and Engineering Bio-Medical Materials and Engineering, IOS Press, 2017, 28 (s1), pp.S75-S79. ⟨10.3233/BME-171626⟩ |
| ISSN: | 0959-2989 1878-3619 |
| Popis: | Selected paper from the 6th China–France International Symposium “Stem Cells and Regenerative Medicine” and the first meeting of the France–China International CNRS Network (GDRI) CeSMeR, Nancy, 10–13 July 2016; International audience; Mesenchymal stem cells (MSCs) are a common tool in regenerative medicine. The nanoscale extracellular vesicles (nEVs) secreted by these cells were recently brought up to light thanks to their therapeutic potential. In this study, we assessed the in vitro behaviour of human umbilical vein endothelial cells (HUVECs) exposed to nEVs derived from human umbilical cord mesenchymal stem cells (hUC-MSCs). Nanoscale extracellular vesicles were isolated and characterized by NanoSight® and flow cytometry. HUVECs were stimulated with various concentrations of nEVs. To assess nEV interactions with HUVECs, confocal microscopy and angiogenesis assay were performed. The use of nEVs derived from hUC-MSCs was able to produce positive outcomes on HUVECs by acting on their angiogenic potential. |
| Databáze: | OpenAIRE |
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