Subcutaneous Administration of Insulin is Associated With Regional Differences in Injection Depot Variability and Kinetics in The Rat
| Autor: | Hanne Hoffmann Frølund Refsgaard, Jens Lykkesfeldt, Per B. Brockhoff, Torben Seested, Anna Katrina Jógvansdóttir Gradel, Trine Porsgaard |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Depot Injections Subcutaneous Endocrinology Diabetes and Metabolism medicine.medical_treatment Iomeprol 030209 endocrinology & metabolism Absorption (skin) Rats Sprague-Dawley Insulin aspart 03 medical and health sciences chemistry.chemical_compound Subcutaneous injection 0302 clinical medicine Endocrinology Pharmacokinetics In vivo Internal medicine Internal Medicine Animals Humans Hypoglycemic Agents Insulin Medicine business.industry X-Ray Microtomography General Medicine Rats 030104 developmental biology chemistry business hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 128:332-338 |
| ISSN: | 1439-3646 0947-7349 |
| Popis: | Background In humans, subcutaneous administration of insulin in the abdominal region or arm is associated with a faster absorption compared to the thigh or buttocks. We hypothesised that this is partly caused by differences in injection depot structure and kinetics and that the variability in insulin exposure differs between injection sites. Material and methods Regional effects on insulin pharmacokinetics were evaluated in a series of studies in Sprague Dawley rats dosed subcutaneously with insulin aspart in the neck or flank. Injection depots were visualised using µCT after subcutaneous dosing with insulin aspart mixed with the contrast agent iomeprol, and insulin exposure was determined between the scans by Luminescent Oxygen Channeling Immunoassay. Results Insulin absorption was significantly delayed by subcutaneous dosing in the flank compared to the neck region (p Conclusion Structure and kinetics of subcutaneous injection depots—as detected by µCT scans—predict insulin exposure and may thus contribute to the regional differences in insulin pharmacokinetics. The present methodology is applicable for visualisation of insulin injection depots in vivo. Our results did however not support a link between the variability in depot size and insulin pharmacokinetics. |
| Databáze: | OpenAIRE |
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