Tumor-derived exosomes are enriched in ΔNp73, which promotes oncogenic potential in acceptor cells and correlates with patient survival
| Autor: | Beatriz Gil-Calderón, Félix Bonilla, Aránzazu García-Grande, Marta Rodríguez, Coral San Millán, Beatriz Soldevilla, Vanesa García, Gemma Domínguez, Rodrigo Fernández-Periañez, Paloma Martín, Javier Silva |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Cell type Cell Biology Exosomes medicine.disease_cause Exosome Mice Cell Line Tumor microRNA Human Umbilical Vein Endothelial Cells Genetics medicine Animals Humans RNA Messenger Molecular Biology Genetics (clinical) Cell Proliferation Cell growth Tumor Suppressor Proteins Nuclear Proteins Tumor Protein p73 General Medicine HCT116 Cells Prognosis Microvesicles Cell biology DNA-Binding Proteins Survival Rate medicine.anatomical_structure Drug Resistance Neoplasm Cell culture Immunology Female Colorectal Neoplasms Carcinogenesis |
| Zdroj: | Human Molecular Genetics. 23:467-478 |
| ISSN: | 1460-2083 0964-6906 |
| Popis: | Tumor-derived exosomes are emerging as local and systemic cell-to-cell mediators of oncogenic information through the horizontal transfer of mRNAs, microRNAs and proteins during tumorigenesis. The exosomal content has been described as biologically active when taken up by the recipient cell. Identifying the specific molecular cargo of exosomes will help to determine their function in specific steps of the tumorigenic process. Here we evaluate whether ΔNp73 is selectively packaged in tumor-derived exosomes, its function in the acceptor cells in vitro and in vivo and its prognosis potential in cancer. ΔNp73 messenger is enriched in tumor-derived exosomes, suggesting its active sorting in these microvesicles. We observed the transmission of this exosome cargo to different cell types and how it confers proliferation potential and chemoresistance to the acceptor cells in vitro and in animal models. Finally, our data support the potential prognostic value of exosomal ΔNp73 in colon cancer patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|