Methanohalophilus profundi sp. nov., a methylotrophic halophilic piezophilic methanogen isolated from a deep hypersaline anoxic basin

Autor: Laurent Toffin, Stéphane L'Haridon, Charlotte Balière, Morgane Chalopin, Erwan Corre, Mohamed Jebbar, Violetta La Cono, Hani Haroun, Michail M. Yakimov, Patricia Pignet, Erwan Roussel
Přispěvatelé: Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (ABIMS), Fédération de recherche de Roscoff (FR2424), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Insitute for Coastal Marine Environment, National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), This study was funded by the European Union program MaCuMBA (grant agreement 311975) and the MAMBA project (FP7-KBBE-2008–226977). Research was supported by UBO, CNRS and Ifremer., European Project: 311975,EC:FP7:KBBE,FP7-KBBE-2012-6-singlestage,MACUMBA(2012), European Project: 226977,EC:FP7:KBBE,FP7-KBBE-2008-2B,MAMBA(2009), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (FR2424), Consiglio Nazionale delle Ricerche (CNR)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Salinity
MESH: Hydrogen-Ion Concentration
Hydrostatic pressure
MESH: Methanol
Applied Microbiology and Biotechnology
Genes
Archaeal
MESH: Methylamines
RNA
Ribosomal
16S
Anaerobiosis
MESH: Phylogeny
Phylogeny
MESH: Methanosarcinaceae
Methanosarcinaceae
0303 health sciences
biology
Strain (chemistry)
Temperature
Hydrogen-Ion Concentration
Methanogen
MESH: Temperature
Halophile
MESH: RNA
Ribosomal
16S
RNA
Bacterial
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Methanosarcinales
Water Microbiology
MESH: RNA
Bacterial
Deep hypersalin anoxic basin (DHAB)
MESH: Mediterranean Sea
Methanohalophilus
Microbiology
MESH: Genes
Archaeal
Methylamines
03 medical and health sciences
MESH: Hydrostatic Pressure
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
MESH: Anaerobiosis
Botany
Hydrostatic Pressure
Mediterranean Sea
Piezophilic
Seawater
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
030306 microbiology
Methanol
MESH: Seawater
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
16S ribosomal RNA
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Water Microbiology
Methylotrophic
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
MESH: Salinity
Zdroj: Systematic And Applied Microbiology (0723-2020) (Elsevier BV), 2020-09, Vol. 43, N. 5, P. 126107 (8p.)
Systematic and Applied Microbiology
Systematic and Applied Microbiology, 2020, 43 (5), pp.126107. ⟨10.1016/j.syapm.2020.126107⟩
Systematic and Applied Microbiology, Elsevier, 2020, 43 (5), pp.126107. ⟨10.1016/j.syapm.2020.126107⟩
ISSN: 0723-2020
DOI: 10.1016/j.syapm.2020.126107⟩
Popis: International audience; A novel anaerobic methylotrophic halophilic methanogen strain SLHTYRO T was isolated from a deep hypersaline anoxic basin called "Tyro" located in the Eastern Mediterranean Sea. Cells of SLHTYRO T were motile cocci. The strain SLHTYRO T grew between 12 and 37 °C (optimum 30 °C), at pH between 6.5 and 8.2 (optimum pH 7.5) and salinity from 45 to 240 g L −1 NaCl (optimum 135 g L −1). Strain SLHTYRO T was methylotrophic methanogen able to use methylated compounds (trimethylamine, dimethylamine, monomethylamine and methanol). Strain SLHTYRO T was able to grow at in situ hydrostatic pressure and temperature conditions (35 MPa, 14 °C). Phylogenetic analysis based on 16S rRNA gene and mcrA gene sequences indicated that strain SLHTYRO T was affiliated to genus Methanohalophilus within the order Methanosarcinales. It shared >99.16% of the 16S rRNA gene sequence similarity with strains of other Methanohalophilus species. Based on ANIb, AAI and dDDH measurements, and the physiological properties of the novel isolate, we propose that strain SLHTYRO T should be classified as a representative of a novel species, for which the name Methanohalophilus profundi sp. nov. is proposed; the type strain is SLHTYRO T (=DSM 108854 = JCM 32768 = UBOCC-M-3308).
Databáze: OpenAIRE
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