Lung pathology and immediate hypersensitivity in a mouse model after vaccination with pertussis vaccines and challenge with Bordetella pertussis

Autor: Jan A. M. A. Dormans, Rob J. Vandebriel, Eric R. Gremmer, Sandra M. M. Hellwig, Jolanda P. Vermeulen, Frits R. Mooi, Paul J.M. Roholl
Rok vydání: 2004
Předmět:
Hypersensitivity
Immediate
Bordetella pertussis
medicine.medical_specialty
Whooping Cough
medicine.medical_treatment
Mice
Inbred Strains
Interferon-gamma
Mice
Vaccines
Acellular
medicine
Animals
Lung
Whooping cough
Administration
Intranasal
Diphtheria-Tetanus-Pertussis Vaccine
Mice
Knockout
Pertussis Vaccine
Mice
Inbred BALB C
Interleukin-13
General Veterinary
General Immunology and Microbiology
biology
medicine.diagnostic_test
business.industry
Tumor Necrosis Factor-alpha
Diphtheria
Public Health
Environmental and Occupational Health
Anatomical pathology
respiratory system
Immunoglobulin E
biology.organism_classification
medicine.disease
Virology
Interleukin-10
Vaccination
Mice
Inbred C57BL
Infectious Diseases
Bronchoalveolar lavage
Immunology
Molecular Medicine
Pertussis vaccine
Female
Interleukin-4
Lymph Nodes
Interleukin-5
business
Adjuvant
medicine.drug
Zdroj: Vaccine. 25(12)
ISSN: 0264-410X
Popis: While evaluating vaccine efficacy against clinical Bordetella pertussis isolates in mice, after challenge vaccinated mice showed increased lung pathology with eosinophilia, compared to challenged, non-vaccinated animals. This led us to study bacterial clearance, lung pathology, lung TNF-alpha expression, and parameters of immediate hypersensitivity (IH), being serum IgE levels, eosinophil numbers in the bronchoalveolar lavage fluid, and ex vivo IL-4, IL-5, IL-10, IL-13, and IFN-gamma production by the bronchial lymph node cells. BALB/c mice received a combined Diphtheria (D), Tetanus (T), Poliomyelitis, and whole-cell Pertussis vaccine (WCV), a combined D, T, and three-component acellular Pertussis vaccine (ACV), aluminium hydroxide adjuvant, or PBS, 28 and 14 days before B. pertussis infection. Similarly treated non-infected mice were taken as a control. Infection induced pathology; this induction was stronger after (especially WCV) vaccination. WCV but not ACV vaccination induced TNF-alpha expression after challenge. After challenge, IH parameters were strongly increased by (especially ACV) vaccination. Vaccinated IL-4 KO mice showed similar clearance and pathology, in the absence of IgE and with reduced numbers of eosinophils. Vaccinated (Th1-deficient) T-bet KO mice showed reduced clearance and similar pathology. In summary, after challenge vaccination increased lung pathology, TNF-alpha expression (only WCV), and IH parameters. Th1 cells were critical for clearance.
Databáze: OpenAIRE
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