Square Membrane Resonators Supporting Degenerate Modes of Vibration for High-Throughput Mass Spectrometry of Single Bacterial Cells
| Autor: | Adrián Sanz-Jiménez, Jose J. Ruz, Eduardo Gil-Santos, Oscar Malvar, Sergio García-López, Priscila M. Kosaka, Álvaro Cano, Montserrat Calleja, Javier Tamayo |
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| Přispěvatelé: | European Commission, European Research Council, Instituto de Salud Carlos III, Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Comunidad de Madrid, Ruz Martínez, José Jaime, Gil-Santos, Eduardo, Malvar, Óscar, García-López, Sergio, Kosaka, Priscila M., Calleja, Montserrat |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
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| Popis: | In nanomechanical mass spectrometry, sensing devices are commonly placed in the vacuum environment and a stream of analytes is directed toward the sensor surface for measurement. Beam structures, such as double-clamped nanobeams and nanocantilevers, are commonly used due to their low inertial mass and the simplicity of the analytical models for mass extraction. The drawback of such structures is their low capture areas, compromising the capture efficiency and throughput of this technique. Bi-axisymmetric resonators, such as ultrathin square or circular membranes, arise as an optimal geometry to maximize capture efficiency while minimizing the device inertial mass. However, these structures present degenerate mechanical modes, whose frequency perturbations upon analyte adsorption are not well described by commonly used models. Furthermore, prior knowledge of the vibration mode shapes of the sensor is crucial for the correct calculation of the analyte’s mass, and the mode shape of degenerate modes may change significantly after every adsorption event. In this work, we present an accurate analytical theory to describe the effect of mass adsorption on the degenerate modes of square membrane resonators and propose two different methods based on the new theory to update the vibration mode shapes after every adsorption event. Finally, we illustrate the problem experimentally obtaining the mass and adsorption position of individual Escherichia coli K-12 bacterial cells on commercial square silicon nitride membranes fabricated with very small tolerances. This work was partially supported by the European Union’s Horizon 2020 Research and Innovation Program under grant agreement no. 731868 VIRUSCAN, by the ERC CoG grant 681275 “LIQUIDMASS”, and by 101034583-H2020-FETOPEN “VIRAIR”. It has received funding from the Spanish Science and Innovation Ministry through projects AES (DTS21/00136/ISCIII/Unión Europea/FEDER), “CELLBIOPHYS” (PID2021-126993OB-I00/MCIN/AEI/ 10.13039/501100011033/FEDER, UE), “ONCOLIGHT” (PDC2022-133503-I00/MCIN/AEI/10.13039/ 501100011033/European Union/Next Generation EU/ PRTR), “ONCODEEPLASM” (PLEC2021-007892/MCIN/ AEI/10.13039/501100011033/European Union/Next Generation EU/PRTR) and by Comunidad Autónoma de Madrid Y2020/BIO-65194 scCANCER-CM. |
| Databáze: | OpenAIRE |
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