The possibility of using citric phosphate dextrose in chilling ram semen instead of egg yolk and soybean lecithin to improve fertility
| Autor: | Mahmoud Yassin Mohamed, Ezz I. Khalifa, Tarek M. M. Mahdy |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
food.ingredient 040301 veterinary sciences media_common.quotation_subject Semen Fertility Biology Semen collection law.invention Phosphates 0403 veterinary science chemistry.chemical_compound food Animal science Cryoprotective Agents Food Animals law Yolk Soybean Lecithin Lecithins Animals media_common Cryopreservation Sheep urogenital system Extender 0402 animal and dairy science 04 agricultural and veterinary sciences Phosphate 040201 dairy & animal science Sperm Egg Yolk Glucose chemistry Sperm Motility Animal Science and Zoology Soybeans Semen Preservation |
| Zdroj: | Journal of animal physiology and animal nutritionREFERENCES. 104(6) |
| ISSN: | 1439-0396 |
| Popis: | Semen chilling has some harmful effects on sperm characteristics. Modification of extenders by using conventional and non-conventional chilling substances can affect sperm characteristics and fertility. One of the important substances using to chilling human blood cells is citric phosphate dextrose (CPD); therefore, it can be used to store ram semen. This study aimed to assay the ability of CPD instead of egg yolk (EGD) and soybean lecithin (SLD)-based extenders to store ram semen at 5°C. This trial was carried on pooling semen samples that were collected from five rams using a standard artificial vagina and an oestrous restrained ewe. Immediately after semen collection, semen was divided into four portions. The EGD, SLD and citric phosphate dextrose with an antibiotic (CPDA) and without antibiotic (CPD) were extender in the first, second, third and fourth portions of semen, respectively. Sperm characteristics were evaluated after dilution and cooling up to 7 days. Also, conception rate and fertility potential were assayed by 48 ewes (12 each) after 2 days of store ram semen extenders. Data clearly indicated that higher (p > .05) sperm characteristics in EGD, SLD and CPDA than CPD at 0 day of storage. Whilst, sperm characteristics from 1 day up to 7 days of storage were higher (p |
| Databáze: | OpenAIRE |
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