Copper chaperone ATOX1 regulates pluripotency factor OCT4 in preimplantation mouse embryos
| Autor: | Emanuele Celauro, Juan Carlos Fierro-González, Amisa Mukaj, Pernilla Wittung-Stafshede |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Pluripotent Stem Cells
0301 basic medicine Biophysics Embryonic Development Biology Biochemistry Mice 03 medical and health sciences 0302 clinical medicine Copper Transport Proteins Transcription (biology) Animals Inner cell mass Gene silencing Cation Transport Proteins Molecular Biology Transcription factor Embryogenesis Gene Expression Regulation Developmental RNA Embryo Cell Biology Molecular biology Cell biology Blastocyst 030104 developmental biology Chaperone (protein) embryonic structures biology.protein Octamer Transcription Factor-3 Copper 030217 neurology & neurosurgery Molecular Chaperones |
| Zdroj: | Biochemical and Biophysical Research Communications. 491:147-153 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2017.07.064 |
| Popis: | Despite of the importance of copper (Cu) during pregnancy, the roles of Cu-binding proteins during early embryonic development are unknown. The Cu chaperone ATOX1 was recently suggested to have additional functions related to transcription and cancer. When we analyzed single-cell RNA transcript data from early mouse embryos, Atox1 transcript levels increased dramatically at the 8-cell stage and, at 16- and 32-cell embryo stages, matched those of Oct4 which expresses a transcription factor essential for pluripotency in the inner cell mass. To explore this, we probed Atox1 expression during the first week of development of mouse embryos. ATOX1 appeared ubiquitously expressed throughout the cells until compaction; in subsequent embryo stages, ATOX1 relocalized to cytoplasmic perinuclear domains in the inner cell mass. Silencing of Oct4 did not affect Atox1 expression, but silencing of Atox1 at the 2-cell stage strongly diminished Oct4 expression in 16-cell embryos. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect