Morphological and neurochemical changes in GABAergic neurons of the aging human inferior colliculus
| Autor: | Tara Sankar Roy, Chaitanya Rama Bai Paltati, Shubhi Saini, Punit Kumar, D.N. Bhardwaj, Tony George Jacob, Indra Pal, Charanjeet Kaur |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Inferior colliculus Aging Auditory Pathways Adolescent Glutamate decarboxylase Stereology Inhibitory postsynaptic potential Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hearing Humans GABAergic Neurons Child Neurotransmitter gamma-Aminobutyric Acid Aged Aged 80 and over Inferior Colliculi Cell Death biology Glutamate Decarboxylase Age Factors Middle Aged Presbycusis Sensory Systems Parvalbumins 030104 developmental biology nervous system chemistry biology.protein GABAergic Female Neuroscience 030217 neurology & neurosurgery Parvalbumin |
| Zdroj: | Hearing Research. 377:318-329 |
| ISSN: | 0378-5955 |
| DOI: | 10.1016/j.heares.2019.02.005 |
| Popis: | It is well known that quality of hearing decreases with increasing age due to changes in the peripheral or central auditory pathway. Along with the decrease in the number of neurons the neurotransmitter profile is also affected in the various parts of the auditory system. Particularly, changes in the inhibitory neurons in the inferior colliculus (IC) are known to affect quality of hearing with aging. To date, there is no information about the status of the inhibitory neurotransmitter GABA in the human IC during aging. We have collected and processed inferior colliculi of persons aged 11-97 years at the time of death for morphometry and immunohistochemical expression of glutamic acid decarboxylase (GAD67) and parvalbumin. We used unbiased stereology to estimate the number of cresyl-violet and immunostained neurons. Quantitative real-time PCR was used to measure the relative expression of the GAD67 mRNA. We found that the number of total, GABAergic and PV-positive neurons significantly decreased with increasing age (p 0.05). The proportion of GAD67-ir neurons to total number of neurons was also negatively associated with increasing age (p = 0.004), but there was no change observed in the proportion of PV-ir neurons relative to GABAergic neurons (p = 0.25). Further, the fold change in the levels of GAD67 mRNA was negatively correlated to age (p = 0.024). We conclude that the poorer quality of hearing with increasing age may be due to decreased expression of inhibitory neurotransmitters and the decline in the number of inhibitory neurons in the IC. |
| Databáze: | OpenAIRE |
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