Expression of Ins1 and Ins2 genes in mouse fetal liver
| Autor: | Tohru Onogawa, Asako Itaya-Hironaka, Akiyo Yamauchi, Maiko Takeda, Hiroshi Okamoto, Hiroyo Ota, Sumiyo Sakuramoto-Tsuchida, Koji Nata, Shoko Murakami-Kawaguchi, Masato Kato, Shin Takasawa |
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| Rok vydání: | 2013 |
| Předmět: |
Transcriptional Activation
endocrine system Maf Transcription Factors Large Histology endocrine system diseases medicine.medical_treatment Electrophoretic Mobility Shift Assay Nerve Tissue Proteins Biology Pathology and Forensic Medicine Mice Transactivation Fetus Pregnancy Basic Helix-Loop-Helix Transcription Factors medicine Animals Insulin RNA Messenger Transcription factor Proinsulin Homeodomain Proteins NeuroD Mice Inbred ICR Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Developmental Promoter Cell Biology Transfection Glucagon Immunohistochemistry Molecular biology Rats Liver embryonic structures Hepatocytes Trans-Activators PDX1 Female Somatostatin |
| Zdroj: | Cell and Tissue Research. 355:303-314 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-013-1741-4 |
| Popis: | A possible cure for diabetes is explored by using non-pancreatic cells such as fetal hepatocytes. The expression of insulin and transcription factors for insulin is investigated in mouse fetal liver. We detected mRNAs for insulin I (Ins1) and insulin II (Ins2) and proinsulin- and mature insulin-positive cells in mouse fetal liver by reverse transcription plus the polymerase chain reaction and immunohistochemistry. Glucagon, somatostatin and pancreatic polypeptide were not expressed throughout development. Mouse Ins2 and Ins1 promoters were transiently activated in mouse fetal hepatocytes of embryonic days 13.5 and 16.5, respectively. Pancreatic and duodenal homeobox 1 (Pdx1) mRNA was not expressed during development of the liver. In contrast, mRNAs and proteins of neurogenic differentiation (NeuroD)/β cell E-box transactivator 2 (Beta2) and v-maf musculoaponeurotic fibrosarcoma oncogene homolog (MafA) were almost simultaneously expressed with insulin genes in the liver. Ins2 and Ins1 promoters were activated in hepatoma cells by the transfection of the expression vector for NeuroD/Beta2 alone and by the combination of NeuroD/Beta2 and MafA, respectively. These results indicate that the expression of NeuroD/Beta2 and MafA is linked temporally with the transcription of Ins2 and Ins1 genes in mouse fetal liver and suggest the potential usage of fetal hepatocytes to make insulin-producing β cells by introducing transcription factors. |
| Databáze: | OpenAIRE |
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