Interleukin-10 inhibits proinflammatory chemokine release by neutrophils of the newborn without suppression of nuclear factor-kappa B
| Autor: | Johny Tryzmel, Ivana Vancurova, Dennis Davidson, Susana Castro-Alcaraz, Veronika Miskolci |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Chemokine Lipopolysaccharide Neutrophils medicine.medical_treatment Stimulation Inflammation Granulocyte Proinflammatory cytokine chemistry.chemical_compound NF-KappaB Inhibitor alpha Internal medicine medicine Humans Chemokine CCL4 Cells Cultured biology business.industry Tumor Necrosis Factor-alpha Interleukin-8 Infant Newborn NF-kappa B Infant Macrophage Inflammatory Proteins Fetal Blood Interleukin-10 medicine.anatomical_structure Endocrinology Cytokine chemistry Pediatrics Perinatology and Child Health biology.protein Tumor necrosis factor alpha I-kappa B Proteins medicine.symptom Chemokines business |
| Zdroj: | Pediatric research. 54(3) |
| ISSN: | 0031-3998 |
| Popis: | An increase in polymorphonuclear leukocytes (PMNs) and proinflammatory chemokines, such as IL-8 and macrophage inflammatory protein-1 alpha (MIP), are found in the airways during early stages of bronchopulmonary dysplasia. We determined whether IL-10 produces a dose-related inhibition of proinflammatory chemokine release from stimulated neutrophils of the newborn and whether the mechanism involves the pivotal transcription factor, nuclear factor-kappa B. PMNs isolated from the cord blood of healthy newborns were stimulated submaximally with either lipopolysaccharide (n = 5) or tumor necrosis factor (n = 4), with and without IL-10 (0.01-1000 ng/mL). IL-8 and MIP release were measured in cell culture supernatants at 18 h. The presence or absence of nuclear factor-kappa B activity and inhibitor-kappa B alpha degradation was measured at 30 min and 3 h after PMN stimulation began. During lipopolysaccharide stimulation, IL-10 significantly reduced IL-8 levels from 50 +/- 16 ng/mL to 7 +/- 3 ng/mL, and MIP levels from 14 +/- 5 to 0.7 +/- 0.1 ng/mL (mean +/- SEM, p0.01). IL-10 produced an insignificant reduction in IL-8 and MIP levels after stimulation of PMNs with tumor necrosis factor. IL-10 did not inhibit nuclear factor-kappa B activation and inhibitor-kappa B alpha degradation in PMNs stimulated with tumor necrosis factor or lipopolysaccharide for 30 min. After PMN stimulation for 3 h, inhibitor-kappa B alpha cytoplasmic levels were restored; however, they were unaffected by IL-10. We conclude that IL-10 is a potent inhibitor of lipopolysaccharide-stimulated release of IL-8 and MIP from neutrophils of the newborn via a mechanism not involving nuclear factor-kappa B activity. Further work is needed to determine whether exogenous IL-10 may be useful for suppressing inflammation in bronchopulmonary dysplasia. |
| Databáze: | OpenAIRE |
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