Analysis of TSG101 tumour susceptibility gene transcripts in cervical and endometrial cancers
Autor: | H. J. Wei, Tsung-Hsien Su, C. P. Chang, C. J. Jeng, Jyh-Chwan Wang, Jan-Gowth Chang, Y. J. Chen |
---|---|
Rok vydání: | 1999 |
Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Tumor suppressor gene Transcription Genetic cervical cancer DNA Mutational Analysis Loss of Heterozygosity Uterine Cervical Neoplasms tumour-suppressor gene Biology medicine.disease_cause Polymerase Chain Reaction Loss of heterozygosity medicine Carcinoma Humans Genes Tumor Suppressor transcripts Cervix Papillomaviridae Aged TSG101 gene TSG101 Gene Endometrial cancer Chromosomes Human Pair 11 Papillomavirus Infections Cancer Regular Article DNA Neoplasm Middle Aged medicine.disease Endometrial Neoplasms medicine.anatomical_structure Cell Transformation Neoplastic Oncology endometrial cancer Cancer research Female Carcinogenesis |
Zdroj: | British Journal of Cancer |
ISSN: | 0007-0920 |
Popis: | Carcinoma of the uterine cervix is a common malignancy among women that has been found to show loss of heterozygosity in the chromosome 11p. Recent studies have localized the TSG101 gene in this region, and also demonstrated a high frequency of abnormalities of this gene in human breast cancer. To determine the role of the TSG101 gene in the carcinogenesis of cervical and uterine carcinoma, 19 cases of cervical carcinoma and five cases of endometrial carcinoma, as well as nearby non-cancerous tissue from the same patients, and 16 blood samples from healthy persons as normal control were analysed by Southern blot analysis of genomic DNA, reverse transcription of the TSG101 mRNA followed by PCR amplification and sequencing of the products. We found that abnormal transcripts of the TSG101 gene were common both in cancerous or non-cancerous tissues of the uterus and cervix and in normal peripheral mononuclear cells. There was no genomic deletion or rearrangement in spite of the presence of abnormal transcripts, and no definite relationship between the abnormal transcripts and HPV infection was found. Although the frequency of abnormal transcripts was higher in cancerous than in non-cancerous tissue, normal peripheral mononuclear cells also had abnormal transcripts. Given these findings, the role of the TSG101 gene as a tumour-suppressor gene should be re-evaluated. Because some aberrant transcripts could be found at the first PCR reaction, we suggest that the aberrant transcripts might be the result of imperfect minor splicesome products. © 1999 Cancer Research Campaign |
Databáze: | OpenAIRE |
Externí odkaz: |