Molecular genetic characterization of different Trypanosoma cruzi strains and comparison of their development in Mus musculus and Calomys callosus
| Autor: | V. Hemleben, W. Engels, C. K. Dost, José Clóvis do Prado, Sergio de Albuquerque |
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| Rok vydání: | 2002 |
| Předmět: |
Lineage (genetic)
Trypanosoma cruzi Parasitemia Polymerase Chain Reaction Mice chemistry.chemical_compound Species Specificity Molecular marker Animals Humans Chagas Disease Genetic variability Genetics Geography General Veterinary biology Strain (biology) Kinetoplastida General Medicine DNA Protozoan Calomys callosus biology.organism_classification RAPD Muridae Disease Models Animal Infectious Diseases chemistry Insect Science Disease Progression Parasitology |
| Zdroj: | Parasitology Research. 88:609-616 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-001-0553-8 |
| Popis: | Trypanosoma cruzi populations are characterized by diverse morphology, heterogeneous biological behavior, high genetic variability, and distinctly different clinical courses. The first objective of this work was to characterize different strains of T. cruzi with various molecular markers [simple-sequence-repeat PCR, randomly amplified polymorphic DNA (RAPD)-PCR, mini-exon genes]. All examined strains could be divided into two major lineages. Only one strain showed a different banding pattern in RAPD-PCR, which could be a further indication of the existence of a third lineage. The second aim was to examine the biological behavior of the different strains. Two animal models, Calomys callosus and Mus musculus, were infected. The results provide strong evidence that the biological behavior of the strains is not only lineage-specific. It appears that all factors, such as the infecting strain belonging to a certain lineage, the predominant morphological form of the isolate, and the immune response of the respective infected host, play an important role in the course of this infection. |
| Databáze: | OpenAIRE |
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