Structural snapshots of the β-barrel assembly machinery
Autor: | Jeremy Bakelar, Susan K. Buchanan, Nicholas Noinaj |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Chemistry food and beverages Cell Biology Biochemistry Article Protein Structure Secondary 03 medical and health sciences Barrel Structure-Activity Relationship 030104 developmental biology Membrane protein Membrane biogenesis Gram-Negative Bacteria Biophysics Protein folding Bacterial outer membrane Molecular Biology Biogenesis Bacterial Outer Membrane Proteins |
Popis: | The β-barrel assembly machinery (BAM) is a multi-component complex responsible for the biogenesis of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria, with conserved systems in both mitochondria and chloroplasts. Given its importance in the integrity of the outer membrane and in the assembly of surface exposed virulence factors, BAM is an attractive therapeutic target against pathogenic bacteria, particularly multi-drug resistant strains. While the mechanism for how BAM functions remains elusive, previous structural studies have described each of the individual components of BAM, offering only a few clues to how the complex functions. Recently, a number of structures have been reported of complexes, including that of fully assembled BAM in differing conformational states. These studies have provided the molecular blueprint detailing the atomic interactions between the components and have revealed new details about BAM, which suggest a dynamic mechanism that may use conformational changes to assist in the biogenesis of new OMPs. |
Databáze: | OpenAIRE |
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