Evaluation of KRAS Gene Expression and LCS6 Variant in Genomic and Cell-Free DNA of Iranian Women With Endometriosis
| Autor: | Maryam Farahani, Soheila Amini Moghaddam, Shirin Shahbazi, Reza Mahdian |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Endometriosis Single-nucleotide polymorphism Iran Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Proto-Oncogene Proteins p21(ras) Germline mutation Risk Factors Proto-Oncogene Proteins microRNA Gene expression medicine Humans Genetic Predisposition to Disease RNA Messenger 3' Untranslated Regions neoplasms Binding Sites Cell-Free System Reverse Transcriptase Polymerase Chain Reaction Obstetrics and Gynecology Original Articles Middle Aged medicine.disease digestive system diseases Up-Regulation MicroRNAs Phenotype Real-time polymerase chain reaction Case-Control Studies ras Proteins Cancer research Biomarker (medicine) Female KRAS |
| Zdroj: | Reproductive Sciences. 22:679-684 |
| ISSN: | 1933-7205 1933-7191 |
| DOI: | 10.1177/1933719114556478 |
| Popis: | Since the activation of KRAS results in de novo endometriosis in mice, KRAS is regarded as a crucial gene in ectopic endometrial implantation. Recently, it has been reported that 31% of women with endometriosis have KRAS let-7 complementary binding site 6 single-nucleotide polymorphism (LCS6 SNP). This study addresses the correlation between KRAS LCS6 SNP and endometriosis in a case–control study. To detect probable somatic mutation in ectopic endometrial tissue, we evaluated LCS6 SNP in cell-free DNA samples. Quantitative real-time reverse transcription-polymerase chain reaction was performed to determine the expression of KRAS transcripts in eutopic endometrial tissue. Our results suggest that the variant is not associated with the development of endometriosis in Iranian women. We observed higher levels of KRAS messenger RNA (mRNA) expression in eutopic endometrium of patients with endometriosis compared to controls. Although, the KRAS LCS6 is neither constitutional nor somatic biomarker for endometriosis, increased expression ratio of KRAS mRNA indicates its role in the implantation of endometrial tissue outside the uterine cavity. |
| Databáze: | OpenAIRE |
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