Analysis of endocrine disruption effect of Roundup® in adrenal gland of male rats
| Autor: | Aparamita Pandey, Medhamurthy Rudraiah |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Glyphosate
Health Toxicology and Mutagenesis Toxicology Sr-b1 scavenger receptor class B member 1 Creb cAMP response element-binding protein Hmgcs 3-hydroxy-3-methylglutaryl-coenzyme A synthase StAR steroidogenic acute regulatory protein chemistry.chemical_compound EIA enzyme Immunoassay Corticosterone Receptor Adrenal gland Adrenal cortex Steroidogenic acute regulatory protein qPCR quantitative real-time PCR EDTA ethylenediaminetetraacetate ELISA enzyme-linked immunosorbent assay Mc2r melanocortin-2 receptor cAMP cyclic adenosine monophosphate medicine.anatomical_structure Endocrine disruptor SDS PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis ACTH adrenocorticotropic hormone EGTA ethylene glycol tetraacetate medicine.medical_specialty StAR RIPA buffer radioimmunoprecipitation assay buffer Ldlr low density lipoprotein receptor Biology TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling Article EDC endocrine disrupting chemical PBS phosphate buffer saline lcsh:RA1190-1270 Internal medicine medicine LD50 lethal dose 50% Cyclic adenosine monophosphate ACTH receptor Protein kinase A lcsh:Toxicology. Poisons L:D cycle light–dark cycle Hmgcr 3-hydroxy-3-methylglutaryl-CoA reductase β ME beta mercaptoethanol TdT terminal deoxynucleotidyl transferase Endocrinology chemistry Steroidogenesis RIA radioimmunoassay Rat PKA protein kinase A Hsl hormone-sensitive lipase DPX distrene plasticiser xylene SD standard deviation DAPI 4′ 6-diamidino-2-phenylindole |
| Zdroj: | Toxicology Reports Toxicology Reports, Vol 2, Iss C, Pp 1075-1085 (2015) |
| ISSN: | 2214-7500 |
| DOI: | 10.1016/j.toxrep.2015.07.021 |
| Popis: | The effect of Roundup® on adrenal gland steroidogenesis and signaling pathway associated with steroid production was investigated. Doses of 10, 50, 100 and 250mg/kg bw/d Roundup® were administered for two weeks to adult male rats. The 10mg/kg bw/d dose which reduced circulatory corticosterone levels, but did not change food consumption and body weight, was selected for further study. The expression of cholesterol receptor (low density lipoprotein receptor), de novo cholesterol synthesis enzyme (3-hydroxy-3-methylglutaryl-coenzyme A synthase), hormone-sensitive lipase, steroidogenic acute regulatory protein (StAR) mRNA and phosphorylated form was decreased. Adrenocorticotropic hormone receptor (ACTH), melanocortin-2 receptor, expression was not changed but circulatory ACTH levels and adrenal cortex protein kinase A (PKA) activity were reduced. Surprisingly, exogenous ACTH treatment rescued steroidogenesis in Roundup®-treated animals. Apoptosis was evident at 250mg/kg bw/d, but not at 10mg/kg bw/d dose. These results suggest that Roundup® may be inhibitory to hypothalamic–pituitary axis leading to reduction in cyclic adenosine monophosphate (cAMP)/PKA pathway, StAR phosphorylation and corticosterone synthesis in the adrenal tissue. |
| Databáze: | OpenAIRE |
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