An L-type lectin receptor-like kinase promotes starch accumulation during rice pollen maturation
| Autor: | Yang Tao, Aiping Zheng, Jun Zhu, Yueyang Liang, Guoqiang Yuan, Xu Zhang, Aijun Wang, Xiumei Yu, Shiquan Wang, Miaomiao Liu, Qiao Xiao, Lingxia Wang, Ting Zou, Qiming Deng, Huainian Liu, Shuangcheng Li, Dan Zhou, Ping Li, Zhiyuan He |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Starch Mutant Biology medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Pollen Lectins medicine Molecular Biology Pollen maturation 030304 developmental biology Plant Proteins chemistry.chemical_classification 0303 health sciences Kinase Phosphotransferases food and beverages Lectin Oryza Cell biology Enzyme chemistry Receptors Mitogen biology.protein Phosphorylation Mutant Proteins 010606 plant biology & botany Developmental Biology |
| Zdroj: | Development (Cambridge, England). 148(6) |
| ISSN: | 1477-9129 |
| Popis: | Starch accumulation is key for the maturity of rice pollen grains; however, the regulatory mechanism underlying this process remains unknown. Here, we have isolated a male-sterile rice mutant, abnormal pollen 1 (ap1), which produces nonviable pollen grains with defective starch accumulation. Functional analysis revealed that AP1 encodes an active L-type lectin receptor-like kinase (L-LecRLK). AP1 is localized to the plasma membrane and its transcript is highly accumulated in pollen during the starch synthesis phase. RNA-seq and phosphoproteomic analysis revealed that the expression/phosphorylation levels of numerous genes/proteins involved in starch and sucrose metabolism pathway were significantly altered in the mutant pollen, including a known rice UDP-glucose pyrophosphorylase (OsUGP2). We further found that AP1 physically interacts with OsUGP2 to elevate its enzymatic activity, likely through targeted phosphorylation. These findings revealed a novel role of L-LecRLK in controlling pollen maturity via modulating sucrose and starch metabolism. |
| Databáze: | OpenAIRE |
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