Long Noncoding RNA Plasmacytoma Variant Translocation 1 (PVT1) Promotes Colon Cancer Progression via Endogenous Sponging miR-26b
| Autor: | Rui Zhang, Jibin Li, Yefu Liu, Jianfeng Zhao, Wenya Li, Wen Shang, Xiaofei Yan, Xin Liu, Keer Jin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Colorectal cancer Carcinogenesis Apoptosis Biology medicine.disease_cause Metastasis 03 medical and health sciences 0302 clinical medicine Gentamicin protection assay Lab/In Vitro Research Cell Movement Cell Line Tumor medicine Humans Neoplasm Invasiveness Cell Proliferation Cell migration General Medicine medicine.disease Long non-coding RNA PVT1 MicroRNAs 030104 developmental biology Tumor progression 030220 oncology & carcinogenesis Colonic Neoplasms Cancer research Disease Progression RNA Long Noncoding |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 1234-1010 |
| Popis: | BACKGROUND Recently, long noncoding RNAs (lncRNAs) have received wide attention in the area of tumor progression. Dysregulation of lncRNAs has been shown to participated in colon cancer, a known malignant tumor. This study aimed to identify the way lncRNA PVT1 affects the progression of colon cancer. MATERIAL AND METHODS Both human colon cancer tissues and 30 paired adjacent normal tissue samples, as well as the colon cancer cells, were collected. Then quantitative real-time (qRT-PCR) was performed to detect the expression of lncRNA PVT1 and miR-26b. Furthermore, the role of PVT1 was determined by function assays such as cell proliferation assay, invasion assay, and wound healing assay. The mechanism was studied using western blot assay and luciferase assay. RESULTS We demonstrate that the expression of PVT1 was significantly higher in tumor tissue compared with the adjacent normal tissue with a lower expression of miR-26b. Moreover, PVT1 promoted tumor growth, migration, and invasion in vitro. In addition, further experiments revealed that miR-26b was a direct target of PVT1 and could inhibit cell migration, invasion, and proliferation in colon cancer. CONCLUSIONS Our results suggest that PVT1 could promote metastasis and proliferation of colon cancer via endogenous sponging and inhibiting the expression of miR-26b, which may highlight the significance of lncRNA PVT1 in colon cancer tumorigenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|