NQO1 activation regulates angiotensin-converting enzyme shedding in spontaneously hypertensive rats
| Autor: | Sung Ho Her, Tae Hwan Kwak, Yong-Hoon Kim, Jung Hwan Hwang, Woo Jin Park, Kyeong Hoon Jeong, Irina V. Balyasnikova, Chul-Ho Lee, Sang Ju Lee, Jung Ran Noh, Gil Tae Gang, Seung Pil Jang, Sang-Woo Kim, Minho Shong, Kyung Shim Kim |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Physiology Blood Pressure Peptidyl-Dipeptidase A Rats Inbred SHR Physiology (medical) Ca2+/calmodulin-dependent protein kinase Internal medicine NAD(P)H Dehydrogenase (Quinone) medicine Animals Humans Phosphorylation Cells Cultured biology Ryanodine receptor Chemistry Angiotensin-converting enzyme Angiotensin II Rats Endocrinology Hypertension biology.protein Calcium NAD+ kinase Calcium-Calmodulin-Dependent Protein Kinase Type 2 Cardiology and Cardiovascular Medicine Homeostasis Intracellular Naphthoquinones |
| Zdroj: | Cardiovascular Research. 99:743-750 |
| ISSN: | 1755-3245 0008-6363 |
| DOI: | 10.1093/cvr/cvt147 |
| Popis: | Aims Angiotensin-converting enzyme (ACE) plays a key role in blood pressure (BP) homeostasis via regulation of angiotensin II. Active ACE ectodomain is enzymatically cleaved and released into body fluids, including plasma, and elevated plasma ACE levels are associated with increased BP. β-lapachone (βL) has been shown to increase cellular NAD+/NADH ratio via activation of NAD(P)H:quinone oxidoreductase 1 (NQO1). In this study, we evaluated whether NQO1 activation by βL modulates BP through regulation of ACE shedding in an animal model of hypertension. Methods and results Spontaneously hypertensive rats (SHR) and a human ACE-overexpressing rat lung microvascular endothelial cell line (RLMVEC-hACE) were used to investigate the mechanism by which βL exerts a hypotensive effect. In vitro studies revealed that βL significantly increased intracellular Ca2+ ([Ca2+]i) levels and CaMKII Thr286 phosphorylation, followed by diminished ACE cleavage secretion into culture media. Inhibition of βL-induced [Ca2+]i level changes through intracellular Ca2+ chelation, Nqo1 -specific siRNA or ryanodine receptor blockade abolished not only βL-induced increase in [Ca2+]i levels and CaMKII phosphorylation, but also βL-mediated decrease in ACE shedding. The effect of βL on ACE shedding was also blocked by inhibition of CaMKII. In SHR, βL reduced BP following increase of CaMKII Thr286 phosphorylation in the lung and decrease of ACE activity and angiotensin II levels in plasma. Conclusion This is the first study demonstrating that ACE shedding is regulated by NQO1 activation, which is possibly correlated with relieving hypertension in SHR. These findings provide strong evidence suggesting that NQO1 might be a new target for ACE modulation and BP control. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|