Crocin Protects Podocytes Against Oxidative Stress and Inflammation Induced by High Glucose Through Inhibition of NF-κB
| Autor: | Jie Lei, Xiaoxia Liu, Puxun Tian, Sutong Li, Junle Yang, Yi Gao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pyrrolidines Physiology Diabetic nephropathy medicine.disease_cause Antioxidants NF-κB lcsh:Physiology Podocyte Crocin chemistry.chemical_compound Mice 0302 clinical medicine Pyrrolidine dithiocarbamate NF-KappaB Inhibitor alpha lcsh:QD415-436 Cell Line Transformed biology lcsh:QP1-981 Podocytes Microfilament Proteins Intracellular Signaling Peptides and Proteins NF-kappa B Drug Synergism Drug Combinations medicine.anatomical_structure 030220 oncology & carcinogenesis Signal Transduction medicine.medical_specialty Nephrin lcsh:Biochemistry 03 medical and health sciences Thiocarbamates Internal medicine medicine Animals WT1 Proteins Adaptor Proteins Signal Transducing Membrane Proteins medicine.disease Carotenoids Cytoskeletal Proteins Oxidative Stress 030104 developmental biology Endocrinology Glucose chemistry Gene Expression Regulation biology.protein Podocin Synaptopodin Oxidative stress |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 42, Iss 4, Pp 1481-1492 (2017) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: Diabetic nephropathy (DN) is a microangiopathic disease characterized by excessive urinary albumin excretion, which occurs in 30% of patients with diabetes mellitus. It is the second leading cause of end-stage renal diseases in China. Nuclear factor-kappa B (NF-κB) is reported to be closely correlated with the inflammation underlying diabetes-associated renal damage. Crocin, a plant-derived compound, has antioxidant properties that may inhibit NF-κB. Methods: In the present study, we used a conditionally immortalized mouse podocyte cell line to explore whether crocin could effectively block albuminuria. Cells were incubated with 15 or 25 mM D-glucose to mimic diabetic conditions. The expression of Wilms tumor 1 (WT-1) and synaptopodin was evaluated to identify differentiated podocytes, and the expression of nephrin, podocin, and CD2ap was measured as markers of slit diaphragms, the main structures within the glomerular filtration barrier. Results: The high-glucose conditions led to reduced nephrin, podocin, and CD2ap expression, which was prevented by pretreatment with crocin. The oxidative stress and pro-inflammatory response of podocytes associated with DN induced by high glucose were also reduced by crocin pretreatment. Phosphorylated IκBα (p-IκBα) expression induced by high glucose was also significantly decreased by crocin pretreatment. Moreover, pyrrolidine dithiocarbamate, a NF-κB inhibitor, pyrrolidine dithio carbamate, augmented the protective effects of crocin. Conclusion: Our results demonstrate a protective role of crocin against damage to podocytes and slit diaphragms under high-glucose conditions via inhibition of NF-κB. This study presents a potential therapy for DN and contributes to the understanding of the mechanism underlying DN. |
| Databáze: | OpenAIRE |
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