A single mutation in capsid protein VP1 (Q145E) of a genogroup C4 strain of human enterovirus 71 generates a mouse-virulent phenotype
Autor: | Zainun Zaini, Peter C. McMinn |
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Rok vydání: | 2012 |
Předmět: |
viruses
Mutant Molecular Sequence Data Clone (cell biology) Mutation Missense Mutagenesis (molecular biology technique) Virulence Biology medicine.disease_cause Virus Mice Virology medicine Enterovirus Infections Animals Humans Point Mutation Amino Acid Sequence Mutation Mice Inbred BALB C Point mutation Enterovirus A Human Phenotype Capsid Mutagenesis Site-Directed Capsid Proteins Sequence Alignment |
Zdroj: | The Journal of general virology. 93(Pt 9) |
ISSN: | 1465-2099 |
Popis: | We modified the capsid protein of a human enterovirus 71 (HEV71) belonging to subgenogroup C4 (HEV71-C4) to generate a mouse virulent strain, based on the genetic information derived from our previous subgenogroup B3 mouse-adapted virus. Infectious clone-derived mutant virus populations containing the capsid protein mutations VP1-Q145E and VP1-Q145G were generated by site-directed mutagenesis of an infectious clone of a subgenogroup C4 strain. Viruses expressing the VP1-Q145E were virulent in 5-day-old BALB/c mice with 100 % mortality rate observed. Skeletal muscle appears to be the primary site of replication of this virus with limb muscle showing severe myositis. Virus was also isolated from spleen, liver, heart and brain of infected mice. This study demonstrates that introducing a key mutation into the HEV71 VP1 capsid protein is able to generate a mouse virulent HEV71 strain from a different genogroup as well as providing an alternative strategy for the generation of mouse virulent HEV71. |
Databáze: | OpenAIRE |
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