Genomic Characterization of hlyF-positive Shiga Toxin-Producing Escherichia coli, Italy and the Netherlands, 2000-2019

Autor: Paola Chiani, Eelco Franz, Federica Gigliucci, Valeria Michelacci, Stefano Morabito, Angela H.A.M. van Hoek, Gaia Scavia, Arnold Knijn, Fabio Minelli
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Serotype
Epidemiology
Shiga toxin–producing Escherichia coli
lcsh:Medicine
medicine.disease_cause
0302 clinical medicine
Plasmid
Genomic Characterization of hlyF-Positive Shiga Toxin–Producing Escherichia coli
Italy and the Netherlands
2000–2019
STX2
030212 general & internal medicine
bacteria
Escherichia coli Infections
Netherlands
ExPEC
Shiga-Toxigenic Escherichia coli
Escherichia coli Proteins
Genomics
foodborne disease
extraintestinal pathogenic E. coli
Europe
STEC
food safety
Infectious Diseases
Italy
whole-genome sequencing
Locus of enterocyte effacement
Microbiology (medical)
Virulence Factors
030231 tropical medicine
Virulence
phylogenesis
Biology
lcsh:Infectious and parasitic diseases
Microbiology
03 medical and health sciences
Antibiotic resistance
medicine
Escherichia coli
Humans
lcsh:RC109-216
antimicrobial resistance
Intimin
bacterial zoonoses
Research
enteric infections
lcsh:R
the Netherlands
bacterial infections and mycoses
zoonoses
hemolytic uremic syndrome
hlyF
Zdroj: Emerging Infectious Diseases
Emerging Infectious Diseases, Vol 27, Iss 3, Pp 853-861 (2021)
Popis: Shiga toxin–producing Escherichia coli (STEC) O80:H2 has emerged in Europe as a cause of hemolytic uremic syndrome associated with bacteremia. STEC O80:H2 harbors the mosaic plasmid pR444_A, which combines several virulence genes, including hlyF and antimicrobial resistance genes. pR444_A is found in some extraintestinal pathogenic E. coli (ExPEC) strains. We identified and characterized 53 STEC strains with ExPEC-associated virulence genes isolated in Italy and the Netherlands during 2000–2019. The isolates belong to 2 major populations: 1 belongs to sequence type 301 and harbors diverse stx2 subtypes, the intimin variant eae-ξ, and pO157-like and pR444_A plasmids; 1 consists of strains belonging to various sequence types, some of which lack the pO157 plasmid, the locus of enterocyte effacement, and the antimicrobial resistance–encoding region. Our results showed that STEC strains harboring ExPEC-associated virulence genes can include multiple serotypes and that the pR444_A plasmid can be acquired and mobilized by STEC strains.
Databáze: OpenAIRE
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