Fast and reliable BIA/amperometric quantification of acetylcysteine using a nanostructured double hydroxide sensor
| Autor: | Juliana S. Bernardes, Carlos A. Neves, Josué M. Gonçalves, Anabel Laza Correa, Koiti Araki, Lúcio Angnes, Pamela O. Rossini |
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| Rok vydání: | 2018 |
| Předmět: |
Analytical chemistry
chemistry.chemical_element 02 engineering and technology Electrocatalyst 01 natural sciences NANOSTRUCTURED ELECTRODE Analytical Chemistry chemistry.chemical_compound X-ray photoelectron spectroscopy NANOPARTICLES Hydroxides Electrodes Detection limit Chemistry 010401 analytical chemistry Ciencias Químicas Pipette AMPEROMETRIC SENSOR Electrochemical Techniques 021001 nanoscience & nanotechnology NICKEL HYDROXIDE Amperometry 0104 chemical sciences Acetylcysteine Nanostructures N-ACETYLCYSTEINE BATCH INJECTION ANALYSIS (BIA) ELECTROCATALYSIS Electrode Flow Injection Analysis Hydroxide ELETROCATÁLISE Química Analítica 0210 nano-technology Tin CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | This study reports the preparation and characterization of nickel/lead hydroxide nanoparticles used to construct electrochemical sensors, which were investigated for amperometric quantification of N-acetylcysteine (NAC). The newly synthesised material presents good uniformity, with the lead (II) ions homogenously incorporated into the alpha nickel hydroxide crystal structure, confirmed by X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy analyses. Films of nanoparticles (3 nm in size) were prepared on conductive fluorine-doped tin oxide-coated glass slides and used connected to a specially built batch injection analysis (BIA) cell with a capacity of only 4 mL and the electrode positioned in the bottom. To attain optimal analytical performance, the main parameters for BIA measurements (volume injected, different velocities of injection and best distance of the pipette from the electrode) were evaluated, as was the working potential, to determine the optimal conditions. Linear responses were obtained for the concentration range from 20 to 220 μmol L−1, and the limits of detection (3σ/slope) and quantification (10σ/slope) were calculated as 0.23 μmol L−1 and 0.70 μmol L−1, respectively. The new NAC sensor does not exhibit a memory effect and has enormous potential utility in the quantitative determination of N-acetylcysteine in drugs. The results of the analysis of NAC obtained using BIA presented good concordance with those obtained by chromatography. The analytical frequency attained using BIA (120 analysis h−1) compares very favourably with the one obtained using chromatography (6 analysis h−1). Fil: Laza Correa, Anabel. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonçalves, Josué M.. Universidade de Sao Paulo; Brasil Fil: Rossini, Pamela O.. Universidade de Sao Paulo; Brasil Fil: Bernardes, Juliana S.. Centro Nacional de Pesquisa Em Energia E Materiais; Brasil Fil: Neves, Carlos A.. Universidade Federal do Pará; Brasil Fil: Araki, Koiti. Universidade de Sao Paulo; Brasil Fil: Angnes, Lucio. Universidade de Sao Paulo; Brasil |
| Databáze: | OpenAIRE |
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