CPE overexpression is correlated with pelvic lymph node metastasis and poor prognosis in patients with early-stage cervical cancer

Autor: Minzhi Hou, Hongwei Shen, Li He, Shanyang He, Jun Liu, Shuzhong Yao, Jianhong Shang, Jin-feng Tan
Rok vydání: 2015
Předmět:
Adult
0301 basic medicine
Oncology
China
medicine.medical_specialty
Pathology
Blotting
Western
Uterine Cervical Neoplasms
medicine.disease_cause
Cervical intraepithelial neoplasia
Polymerase Chain Reaction
Pelvis
Metastasis
03 medical and health sciences
0302 clinical medicine
Internal medicine
Biomarkers
Tumor
medicine
Adjuvant therapy
Humans
Aged
Neoplasm Staging
Cervical cancer
business.industry
Carboxypeptidase H
Obstetrics and Gynecology
General Medicine
Middle Aged
biochemical phenomena
metabolism
and nutrition
Prognosis
Uterine Cervical Dysplasia
medicine.disease
Immunohistochemistry
030104 developmental biology
Real-time polymerase chain reaction
Lymphatic Metastasis
030220 oncology & carcinogenesis
Biomarker (medicine)
Female
Lymph Nodes
Neoplasm Recurrence
Local
Carcinogenesis
business
Zdroj: Archives of Gynecology and Obstetrics. 294:333-342
ISSN: 1432-0711
0932-0067
DOI: 10.1007/s00404-015-3985-6
Popis: Elevated carboxypeptidase E (CPE) levels play crucial roles in tumorigenesis and metastasis. This study investigated the expression and clinicopathological significance of CPE in early-stage cervical cancer. Elevated carboxypeptidase E expression was analyzed using quantitative polymerase chain reaction and western blotting in normal cervical tissue, cervical cancer cell lines, and in cervical cancer tissues and adjacent noncancerous tissues (ANTs) from the same patient. Immunohistochemistry (IHC) was used to examine CPE expression in tissue samples from 112 patients with early-stage cervical cancer (FIGO stages Ia2–IIa2), 60 patients with cervical intraepithelial neoplasia, and 19 patients with normal cervical tissues (NCTs). Associations between CPE expression and prognostic and diagnostic factors were evaluated statistically. CPE expression was significantly higher in cervical cancer cell lines and tissues than in normal tissues and ANTs. Semi-quantitative analysis of IHC indicated that CPE gradually increased from CIN I to cervical cancer, but was absent in NCTs. CPE expression was seen in 40.2 % (45/112) of the cervical cancer samples. CPE expression was significantly associated with FIGO stage (P = 0.003), tumor size (P = 0.012), stromal invasion (P
Databáze: OpenAIRE
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