Highly efficient capture of cancer cells expressing EGFR by microfluidic methods based on antigen-antibody association
| Autor: | Takuya Nagata, Takashi Ohnaga, Yoshinori Takei, Yutaka Shimada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microfluidics Cell lcsh:Medicine Antibodies Monoclonal Humanized Article 03 medical and health sciences 0302 clinical medicine Antigen Cell Line Tumor Neoplasms medicine Humans Epidermal growth factor receptor lcsh:Science Multidisciplinary Cetuximab biology Chemistry lcsh:R Liquid Biopsy Neoplastic Cells Circulating Cell biology ErbB Receptors 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Monoclonal Cancer cell biology.protein lcsh:Q Antibody medicine.drug |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-7 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Epidermal growth factor receptor (EGFR) was evaluated as a target antigen for cancer cell capture by microfluidic methods based on antigen-antibody association. A polymer CTC-chip microfluidic device was surface-functionalized with three different anti-EGFR antibodies and used to capture EGFR-expressing cancer cells. Capture efficacy depended on the type of antibody used, and cetuximab efficiently captured cancer cell lines that had a wide range of EGFR expression. Capture efficiency was analyzed from the viewpoint of antigen-antibody association in a kinetic process, i.e., cell rolling well-known in leukocyte adhesion, and antibodies with a smaller dissociation constant were shown to result in more efficient capture. Moreover, a lower limit of cellular EGFR expression level for the capture was estimated and methods to decrease the limit were discussed based on densities of anti-EGFR antibody on the device surface. |
| Databáze: | OpenAIRE |
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