General characteristics of the influence of surfactants on the bacteriolytic activity of lysozyme based on the example of enzymatic lysis of Lactobacillus plantarum cells in the presence of Tween 21 and SDS
| Autor: | P. A. Levashov, S. A. Smirnov, Wen-Jia Lu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Lysis Kinetics Biophysics Polysorbates Sodium Dodecyl Sulfate Cell Biology Plateau (mathematics) Biochemistry Bacterial cell structure chemistry.chemical_compound Surface-Active Agents Enzyme Pulmonary surfactant chemistry Muramidase Binding site Lysozyme Molecular Biology Hydrophobic and Hydrophilic Interactions Lactobacillus plantarum Protein Binding |
| Zdroj: | Biochemical and biophysical research communications. 575 |
| ISSN: | 1090-2104 |
| Popis: | The general characteristics of the effect of surfactants on the activity of lysozyme were demonstrated. The kinetics of bacterial cell lysis is consistent with the Michaelis-Menten equation and the presence of surfactants does not shift the pH-optimum of activity. Surfactants do not change the Km value but instead, affect the Vmax value. The experimental dependencies are well described by theoretical equations, which assume three surfactant binding sites on the lysozyme molecule. The dependencies of the activity of lysozyme on the surfactant concentration are either a step type (i.e., a higher plateau becomes a lower plateau), or a dependency with a maximum and continuation of the curve in the form of a plateau but with an increase in the surfactant concentration. It can be assumed that there is a mechanism for the regulation of lysozyme activity by an unknown natural factor that has a suitable hydrophobic radical capable of binding to the surface of lysozyme. |
| Databáze: | OpenAIRE |
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