Assessment of anticholinesterase and antioxidant properties of the extracts and (+)-catechin obtained from Arceuthobium oxycedri (D.C.) M. Bieb (dwarf mistletoe)

Autor: Ipek Süntar, E. Küpeli Akkol, Erdem Yesilada, I. Erdogan Orhan
Přispěvatelé: Orhan, IE, Akkol, EK, Suntar, I, Yesilada, E, Yeditepe Üniversitesi
Rok vydání: 2019
Předmět:
Zdroj: South African Journal of Botany. 120:309-312
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2018.09.023
Popis: The hexane, chloroform, ethyl acetate, water, and n-butanol extracts of Arcethobium oxycedri (DC.) M. Bieb. (Santalaceae) were evaluated for their inhibitory effects against acetyl-(AChE) and butyrylcholinesterase (BChE), related to Alzheimer's disease at 200 mu g/mL using microplate assays. Since oxidative stress contributes much to neurodegeneration, antioxidative effect of the extracts was determined in metal-chelating and ferric-reducing antioxidant power (FRAP) test systems as well as antiradical effect against 2,2-diphenyl-1picrylhydrazyl (DPPH). Total phenol and flavonoid contents of the extracts were calculated spectrophotometrically. Our findings indicated that the EtOAc extract, the richest one in total phenol and flavonoid amount (455.22 +/- 4.35 and 194.45 mg/g extract, respectively) displayed the highest cholinesterase inhibition (45.37 +/- 1.23% for AChE and 94.74 +/- 2.43% for BChE at 200 mu g/mL). Fractionation of the EtOAc led to isolation of (+)-catechin as the major compound. Catechin exerted remarkable antioxidant effect, whereas it did not cause cholinesterase inhibition. Therefore, the EtOAc extract deserves further investigation for its high BChE inhibition. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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