| Autor: |
Alan S. Wright, Lathan Ball, Nico J. van Sittert, Paul Aston |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 2(1) |
| ISSN: |
1354-750X |
| Popis: |
A carefully designed hapten-protein conjugate has enabled the generation of a monoclonal antibody reactive with S-phenylmercapturic acid (S-PMA). The immobilized antibody retains immunoreactivity and can be used to enrich S-PMA from the urine of workers exposed to benzene. The performance of the immunoaffinity column has been validated by comparison with data obtained by GC/MS analysis from the urine of benzene-exposed workers (range 12-168 μgl(-1), corr. coeff. 0.98, n = 23). Furthermore immunoaffinity chrom atography facilitates the quantitative determination of urinary S-PMA by reversed phase HPLC. Bioconcentration of S-PMA from the urine of benzeneexposed workers has perm itted the quantification of S-PMA by HPLC at 8 h TWA (time weighted average) exposures of around 1 ppm. The potential application of immunoaffinity enrichment in biomonitoring studies is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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