Activation-induced chromatin reorganization in neurons depends on HDAC1 activity
| Autor: | Agnieszka Grabowska, Hanna Sas-Nowosielska, Bartosz Wojtas, Dagmara Holm-Kaczmarek, Elzbieta Januszewicz, Yana Yushkevich, Iwona Czaban, Pawel Trzaskoma, Katarzyna Krawczyk, Bartlomiej Gielniewski, Ana Martin-Gonzalez, Robert Kuba Filipkowski, Krzysztof Hubert Olszynski, Tytus Bernas, Andrzej Antoni Szczepankiewicz, Malgorzata Alicja Sliwinska, Tambudzai Kanhema, Clive R. Bramham, Grzegorz Bokota, Dariusz Plewczynski, Grzegorz Marek Wilczynski, Adriana Magalska |
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| Přispěvatelé: | National Science Centre (Poland), Foundation for Polish Science, European Commission |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Neurons
Transcription Genetic Long-Term Potentiation Histone Deacetylase 1 Chromatin condensation Chromosomes Mammalian Hippocampus General Biochemistry Genetics and Molecular Biology Chromatin HDAC1 Mice Inbred C57BL Nuclear bodies Chromatin organization Animals Neuronal activation Epigenetics Calcium Signaling Rats Wistar Energy Metabolism |
| Zdroj: | Cell reports |
| Popis: | Spatial chromatin organization is crucial for transcriptional regulation and might be particularly important in neurons since they dramatically change their transcriptome in response to external stimuli. We show that stimulation of neurons causes condensation of large chromatin domains. This phenomenon can be observed in vitro in cultured rat hippocampal neurons as well as in vivo in the amygdala and hippocampal neurons. Activity-induced chromatin condensation is an active, rapid, energy-dependent, and reversible process. It involves calcium-dependent pathways but is independent of active transcription. It is accompanied by the redistribution of posttranslational histone modifications and rearrangements in the spatial organization of chromosome territories. Moreover, it leads to the reorganization of nuclear speckles and active domains located in their proximity. Finally, we find that the histone deacetylase HDAC1 is the key regulator of this process. Our results suggest that HDAC1-dependent chromatin reorganization constitutes an important level of transcriptional regulation in neurons. This work was supported by the National Science Centre grant nos. UMO-2015/18/E/NZ3/00730 (A.M., A.G., H.S.N., E.J. and Y.Y.), 2014/15/N/NZ2/00379 and 2017/24/T/NZ2/00307 (P.T.), 2019/35/O/ST6/02484 (D.P. and G.B.), and 2014/14/M/NZ4/00561 (K.H.O. and R.K.F.). B.W. and B.G. were supported by the Foundation for Polish Science TEAM-TECH Core Facility project “NGS platform for comprehensive diagnostics and personalized therapy in neuro-oncology,” Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund (TEAM to D.P.). A.M.G. was supported by the H2020-MSCA-COFUND-2014 grant Bio4Med (GA no. 665735). |
| Databáze: | OpenAIRE |
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